Screening And Preliminary Application Of Peptide Epitope Of Peste Des Petits Ruminants Virus

PPRV belongs to Paramyxoviridae Morbillivirus.Virus particles contain six structural proteins,which are nucleocapsid protein N,matrix protein M,fusion protein F,hemagglutinin protein H,and phosphoprotein protein P.Large protein L.Biological information of epitope antigens are rich and of great value in the diagnosis of diseases and the development of new vaccines.At present,the epitopes of PPRV are mainly focused on N protein and H protein.In this study,PPRV Nigeria 75/1 strain was used as the reference sequence,using the peptide chip technology,the complete gene sequences of PPRV N,M,H,and F structural proteins were selected to synthesize polypeptide fragments,PPRV negative and positive serum was used for antigen epitopes.Screened and analyzed the obtained data for response rate correlation,stability,three-mode.In this study,39 peptide candidate peptides were screened using peptide chip technology.They were N1,N38,N39,N40,N41,N42,N45,N46,N47,N48,N49,N50,F9~F19,F40,F41,F42,F45,F46,F52,F53,F54,M1,M22,M23,M26,M27,M32,H1,H36,H37.Using R language software to arrange and optimize candidate peptides to obtain the two best combinations.Compared with the gold standard(neutralization test),the combined sensitivity,specificity,and accuracy of N1,N47,N48,N49,N50,F18 and H36 all reached 92%,of N1,N41,F41 and H37,reached 80%,91%,and 86% respectively and can provide theory support for the establishment of the PPRV peptide chip antibody detection technique..Using the VNT test,c-ELISA test,and PPRV peptide chip,the rule of antibody length of PPRV serum was studied.By comparison,the body produced antibodies against N protein earlier than neutralizing antibodies;N46,N47,N48,N49,F9 and F10 can provide technical support for the establishment of the PPRV vaccine immune effect evaluation method.