To Research The Effective Parts Of Pomegranate Peel Polyphenols On Preparation And Stability

Objective:1.Complete the research reports of Kashi region pomegranate resources.2.Make a preliminary study on pilot-scale process to ascertain the production process.3. Complete the accelerated test and long-term testing on Effective Fraction of Polyphenols of Pomegranate Peel.4. Preparative separation of punicalagin and Ellagic acid from Effective Fraction of Polyphenols of Pomegranate Peel by high-speed countercurrent chromatography. Methods:1. Make field surveys to understand the cultivation and processing status of Kashgar Region pomegranate resources.2. Uses consistent with the production of basic conditions for process scale-up studies.3. The stability was studied by the classical constant temperature acceleration experiment and the punicalagin was extracted typically. The reacting speed K25at25℃was calculated according to Arrhenius index law and the effective period of the preparation was calculated. Adopt accelerated test and long-term testing to exam the content of three effective constituents under the influence vary with time, get through test to set validity of crude drug.4.Use HS CCC to preparative separation of punicalagin and Ellagic acid. Results:1.Complete the research report.2. Successful implementation of the effective part of the preparation process from the laboratory to production scale changes.3.The K25of punicalagin=7.8223×10-6, the effective time was the0.9=1.54year. This result consists with the result of accelerated and long-term testing.4. Obtain a high purity punicalagins; ellagic acid by HSCCC, the purity was92%,80%.Conclusion:1.Preliminary understanding of the general situation of Kashgar pomegranate resources.2. Preparation is relative stability, good reproducibility.3.The establishment of the classical constant temperature acceleration experiment is practical for quick determination of the effective time and shortening the developing time of new drugs. The stability of effective fraction is good, valid tentatively scheduled for1.5years.4Obtained high purity punicalagins and ellagic acid by HSCCC.