| Chinese herbal formulae also named as recipe are the main means to treat disease by doctor of traditional Chinese medicine and the study on chemical component of Chinese herbal formulae are the key problem to research Chinese herbal formulae. To understand the substance producing theroputic effect fully is of significance to explore new drugs, to explain the mechanism of medicines compatility and to deepen the cognition of biological science. In this paper, the effective fraction was thought as the core of study on Chinese herbal formulae, and a new viewpoint was proposed, i. e, the group of effective components is the smallest molecule group obtained by modern separation means and still maitaining the theroputic effect of original Chinese herbal formulae. In addition, it was also thought that penetrating into the effective fraction would lay a foundation for realizing the deepening of TCM theory from medicines compatility to chemical components compatility. On the bases of these viewpoints, a Chinese herbal formula---Xuetangning was sysmetically studied to separate the effective fractionguided by the hypoglycemic effect. Main works are as follows:Firstly, the RP-HPLC determination method of alkaloids in Xuetangningwas established, and the extraction process of Xuetangning was optimized?using orthogonal experimental design. In addition, HPCE was used to compare the influence of different drying method on the components and characterinof Xuetangning. In the second chapter, the elution conditions of Rhizoma Coptis extraction from macroporous resin were optimized using L9(3)4 orthogonal experimental design and the influencing factors on the absorption efficiency was explored. In addition, Xuetangning fractions desorpted from macroporous resin were prepared and analysed. In the third chapter, alloxan diabetic mice model was made and the influencing factors on the succeeding rate such as dosage of alloxan and fasting time were explored and the effective fraction of Xuetangning. was verified. In the forth chapter, the quality standard of Xuetangning capsule was drawn up, and the stability of drug material and Xuetangning was studied. In the last chapter, the finger-printings of Coptis chiensis Franch, fraction III and fraction III2 of Xuetangning preparation were made by HPCE and the Bayes identification model which could identify fraction III of Xuetangning and its modified formulae accurately was established. The finger-printing of components in urine samples of rat after administration with Coptis chiensis Franch decocta was also studied.In a word, the effective fraction of Xuetangning was verified, and its determination, finger-printing, theroputic effect and quality control were studied thoroughly in this paper. Results showed that alkaloids are the main material base for descending serum glucose in alloxan diabetic mice. The data of experiment would evolve the relevant materials for new drugs, so considerable social and economic benefit would be created. At the same time these data would provide raw materil and fresh evidence to reveal the group of effective components and to deepen the cognition of TCM compatil i ty mechenism.
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