Effects Of Mitochondrial Fission In Cell Proliferation, Apoptosis And Invasion Of Thyroid Squamous Cell Carcinoma Cell Line SW579

ObjectiveTo detect the expression of Mitofusin 2 (Mfn2) and Dynamin-related proteini (Drp1) which are mitochondrial dynamics proteins in thyroid squamous cell carcinoma cell line SW579 and the effects of Mitochondrial division inhibitor (Mdivi-1) on cell proliferation, apoptosis and invasion of SW579.MethodsIn SW579 and Nthy-ori 3-1 cell lines, the expression levels of Mfn2 and Drp1 were determined by western blot while the transcription level of Mfn2 and Drp1 mRNA were measured by RT-PCR. Then,SW579 cells were divided into control group (DMSO,0.1%) and Mdivi-1 of low, medium and high dose groups (different concentrations of Mdivi-115,30 and 45 ?mol/L were incubated with cells for 16 hours respectively). Then the ability of cell proliferation in each group was detected using MTT assay, the mitochondrial membrane potential in each group was determined by fluorescence spectrophotometer, the expression levels of Caspase-3 were quantified by Western blot and the transcription level of the Caspase-3 mRNA were determined by RT-PCR. The ability of invasion in each group was measured with Transwell assays.Results1. Results Compared with Nthy-ori 3-1, the mRNA transcription and protein expression levels of the Mfn2 was remarkably decreased, while the mRNA transcription and protein expression of the Drp1 was significantly increased in SW579 cells (P<0.01).2. Compared with the control group, the SW579 cell survival rates.mitochondrial membrane potential and the capability of invasion in low, medium and high dose groups of Mdivi-1 were decreased dramatically (P< 0.01).3. Compared with the control group, the mRNA transcription and protein expression of the Caspase-3 in SW579 cell were increased dramatically in low, medium and high dose groups of Mdivi-1 (P<0.01).Conclusions1. Abnormal mitochondrial dynamics may be involved in thyroid squamous cell carcinoma SW579 cells.2.Mdivi-1 can inhibit the cell proliferation and invasion as well as induce apoptosis.