The Synthesis Of Silicon(?) Phthalocyanine Dyes And Application In Photodynamic Therapy

Photodynamic Therapy (PDT), as a kind of high efficient and practical new method for the treatment of maligant tumor and other diseases, more and more causes the attention of people, and the efficiency of singlet oxygen generated by the photosensitizer is the key factor of PDT. It is considerable interesting in Silicon(VI) phthalocyanines(SiPcs) axially modified for being used as photosensitizers. Its advantage is that the introduction of axial ligands can effectively prevent its own accumulation, and can improve its solubility in biological tissues, making the photodynamic effect improved significantly. However, the effects of axial groups of SiPcs on their photodynamic activities have not received much attention and remains little studied.Therefore, we synthesised the silicon phthalocyanine complexes, Pc1 and Pc2, axially modified by hydrophilic groups in this paper. We have improved the existing methods for the synthesis of phthalocyanine. In the synthesis experiment of dichloro silicon phthalocyanine, the solvent was brought quickly to reflux and then the crude product was precipitated in methanol and washed in soxhlet extraction with mixed solvent, the yield can reach to 67.8%.The spectral properties and in vitro photodynamic assay show that they can dissolved well in solvents and the MTT assay also show that the two phthalocyanine compounds own very low toxicity. So Pc1 and Pc2 are accord with the requirement of photosensitizers in the biological application. In vitro photodynamic activity assays, when the concentration of compounds increases to 2?M, the cell survival rate is lower than 20%, at the same time, the PDT efficacies of Pc2 is better than Pc1. In conclusion, we synthesised two phthalocyanine compounds which have certain photodynamic therapy effects. In addition, the photodynamic therapy activity of compound Pc1 and Pc2 are significant higher than that of the typtical photosensitizer, chlorin E6.