| Objective:To investigate the effects and mechanisms of lipoxin A4(LXA4)in attenuating hypoxia/reoxygenation(H/R)injury in human renal tubular epithelial cells(HK-2).Methods:HK-2 cells were exposed to hypoxia followed by reoxygenation with pretreatment of LXA4.Then the cell viability,y-GT,NAG and LAP levels,SOD activity and MDA level were determined.The expressions of mRNA and protein of PPARy and HO-1 were measured using real-time polymerase chain reaction(PCR)and Western blot,respectively.The expressions of HO-1 and Nrf2 were detected by using RNA interference technology to interference PPARy expression.Results:Pretreatment with LXA4 increased the cell viability and SOD activity,and reduced the y-GT,NAG,LAP and MDA levels.However,HO-1 inhibition by ZnPP and siRNA of PPARty abolished the protective role of LXA4 on the cells undergoing hypoxia/reoxygenation injury.Furthermore;the expressions of HO-1,PPARy and Nrf2 were increased in the cells pretreated with LXA4 significantly,whereas these overexpressions of HO-1 and Nrf2 were partly blocked by treatment with siRNA of PPARy.Conclusion:This study revealed that LXA4 pretreatment serves a protective role against hypoxia/reoxygenation injury of human renal tubular epithelial cells via over-expression of HO-1 and activation of PPARy/Nrf2. |
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