The Protective Effect And Its Mechanism Of Hydrogen Sulfide On Diabetic Cardiomyopathy In Diabetic Rats

objective: to investigate whether exogenous hydrogen sulfide can improve cardiac function and reverse ventricular remodeling in diabetic cardiomyopathy rats,and to explore the mechanism of action of exogenous hydrogen sulfide.Methods: the diabetic rats were induced by intraperitoneal injection of streptozotocin.Interfered group was given NaHS,which in aqueous solution releases H2 S,30?mol/L concentration in drinking water for 12 weeks.Cardiac structure and function were evaluated by doppler echocardiography.Employed 18F-2-deoxy-2-fluoro-D-glucose(18F-FDG)small animal positron emission computed tomography imaging(PET/CT)to assess glucose uptake in myocardial cells in diabetic cardiomyopathy rats.Histological changes of hearts were determined by optical microscope.At the same time,using the most advanced gene chip technology and real-time quantitative PCR technology to screen and verify the left ventricular tissue miRNA.Results: exogenous hydrogen sulfide can improve cardiac systolic and diastolic function,reduce cardiomyocyte hypertrophy,interstitial fibrosis and apoptosis,but can not effectively improve the cardiac glucose uptake.Exogenous hydrogen sulfide can down-regulate the expression of let-7i-3p and up-regulate the expression of miR-195-3p.Conclusion: exogenous hydrogen sulfide alleviates the development of diabetic myocardial damage and improves heart failure via attenuation of myocardial hypertrophy,apoptosis and fibrosis.The effect of exogenous hydrogen sulfide on glucose uptake of myocardial cells in diabeticcardiomyopathy may not be the main way to affect cardiac function.These protective effects of exogenous hydrogen sulfide may be related to the down-regulation of miR-195-3p expression and up regulation of let-7i-3p expression.