VEGF Effects On Coagulation And Differentiation Of Blood Cell

Vascular endothelial growth factor?VEGF?is a glycoprotein with molecular weight of 40 KD.It was named vascular permeability factor?VPF?primitively.With the in-depth research,we found that it can promotethe vascular endothelial cell proliferation and migration of endothelial cells and endothelial cell survival,so it is defined as a vascular endothelial growth factor?VEGF?.VEGF familly includes VEGF-A,VEGF-B,VEGF-C,VEGF-D,VEGF-E and Placental growth factor?PIGF?.We often use VEGF replaces VEGF-A.VEGF uses three high affinity of VEGF receptor tyrosine kinase receptor 1?VEGFR1,PLT-1?,receptor 2?KDR/VEGFR2,Flk1?and receptor 3?VEGFR3,PLT-4?transfer all kinds of signaling molecules and participates in cells of the biology of a variety of biochemical reactions.In embryonic period,vascular endothelial growth factor can promote vascular endothelial cell proliferation,migration and the formation of blood vessels,increase the permeability of blood vessel endothelium,directly involved in angiogenesis and development;In adult individuals,VEGF is mainly involved in maintaining normal vascular structures,and adjust the pathological and physiological angiogenesis.With the in-depth study of vascular endothelial growth factor's function,we found that VEGF besides above functions,VEGF also has a significant effecton on blood cell differentiation and blood coagulation process.Previous studies have found that through tetracycline reversible control model that whole body inhibiting the expression of VEGF,we found that obvious changes in blood components,including red blood cells?RBC?and hemoglobin?HGB?ratio increases about 30%,the platelet?PLT?by about 50%.Platelets carrys a large number of VEGF protein,whether directly affect blood coagulation process,there is no literature reports at present.According to reports inhibiting VEGF expression in bone marrow will result in spleen megakaryocyte's number increase.So VEGF may regulate megakaryocyte proliferation,platelet mature and platelet secrete secretion.Platelet factor 4?PF4?expresses on megakaryocyte and platelet specifically,PF4 gene promoter of rats was often used as amegakaryocyte and platelet specific transgenic expression,this experiment using PF4 promoter constructs tetracycline rttR-Krab repressor protein transgenic mice,then hybridizing with VEGF^tetO transgenic mice,feeding doxycycline and removed can regulate the expression of VEGF in megakaryocyte and platelet reversibly.Researching VEGF expression of megakaryocyte and platelet in cells differentiation and blood coagulation balance,the results may provide important theoretical guidance about blood disease.