| To study the changes of sperm motion parameters and sperm nuclear DNA fragmentation index (DFI) in asthenozoospermia patients, and to explore the relationships between progressive motility (PR), sperm motility, sperm velocity and sperm trajectory parameters, to analyze correlation relationship between sperm DNA fragmentation index, semen parameters, and motion parameters.Methods 41 asthenozoospermia cases(sperm concentration?15 ×106/ml, PR<32%) and 48 normal vitality male infertility cases(sperm concentration?15 ×106/ml, PR>32%) were collected, semen routine inspection and computer-aided detection system were performed to detect sperm velocity and trajectory characteristic parameters. Acridine orange staining were used to detect sperm nuclear DNA fragmentation index in all 89 cases of male infertility patients. Diff-quick stain were use to disposed semen samples, and then computer-aided sperm morphology analysis system was used to analysis 200 or more sperms, the results were deal with manual correction. At last, semen parameters, motion parameters, sperm nuclear DNA fragmentation index (DFI) in asthenozoospermia patients and male infertility patients were analyzed and compared. Linear regression analysis was used to analyze progressive motility (PR), sperm motility, sperm motility speed and trajectory parameters of asthenozoospermia group and normal group, correlation analysis was used to explore the relationships between sperm nuclear DNA fragmentation indexes (DFI), semen parameters and motion parameters.Results Asthenospermia sperm concentration was 69.0±49.1×106/ml, the total number of sperm 203±159×106,sperm activity rate 60.8±15.7%, progressive motility(PR) 21.1±8.0%, average path velocity (VAP) 10.7±2.8?m/s?curvilinear velocity (VCL) 20.6±4.9?m/s?rectilinear velocity(VSL) 6.3±2.2?m/s?linearity (LIN) 29.9±8.1%. wobble (WOB) 51.4±5.8%?straightness (STR) 57.2±10.40%?amplitude of lateral head displacement (ALH) 0.53±0.12?m?beat-cross frequency(BCF) 3.20±0.85Hz and the mean angular displacement (MAD) 14.2±3.2°,These parameters were significantly 10wet than that of normal group,sperm concentration was 98.8±38.2×106/ml(P< 0.05).the total number of sperm 281±171×106(P<0.05).sperm activity rate 82.0±7.9%(P<0.01).PR 45.1±8.5%(P<0.01).VAP 18.5±3.0?m/s(P<0.01). VCL 30.7±5.3?m/s(P<0.01).VSL 12.0±2.3?m/s(P<0.01).LIN 39.6±7.0%(P <0.01).WOB 60.5±5.0%(P<0.01).STR 65.1±7.2%(P<0.01).ALH 0.72±0.13?m (P<0.01).BCF 5.07±0.81Hz(P<0.01)and MAD 17.8±2.50(P<0.01).But asthenozoospermia DNA fragmentation index(DFI) was 17.9±12.5%,significantly higher than that of normal group 8.5±6.4%(P<0.01).In Asthenozoospermia group,the correlation coefficient of PR and sperm motility, VAP,VCL,VSL,ALH,LIN,WOB,MAD,BCF,STR were 0.753,0.857,0.632,0.950, 0.521,0.813,0.840,0.578,0.727,0.781,linear regression equation was y=1.480x+ 29.505,y=0.304x+4.267,y=0.383x+12.529,y=0.261x+0.748,y=0.008x+ 0.369,y=0.823x+12.554,y=0.613x+38.505,y=0.231x+9.270,y=0.078x+ 1.563,y=1.010x+35.866,correlation analysis showed there were a significant difference(P<0.001)?In normal vitality group,the correlation coefficient of PR and sperm motility,VAP,VCL,VSL,ALH,LIN,WOB,BCF 0.571,0.868,0.632,0.843, 0.440,0.319,0.431,0.682.Linear regression equation was y=0.531x+58.051,y= 0.311x+4.511,y=-0.393x+13.030,y=0.228x+1.765,y=0.007x+0.407,y= 0.264x+27.695,y=0.254x+49.043,y=0.065x+2.130,correlation analysis showed there were a significant difference(P<0.05),while the correlation coefficient of PR with STR and MAD was 0.182,0.271,linear regression equation was y=0.154x+ 58.102,y=0.080x+14.243,correlation analysis showed there were no statistical significance(P>0.05).In Asthenozoospermia group,correlation coefficients of DFI and PR VAP,VCL, VSL,BCF's were -0.423,-0.380,-0.335,-0.374,-0.401,correlation analysis showed there were statistical significance among them(P<0.05)?and correlation coefficients of DFI and ages,sperm concentration,semen volume,total sperm counts,sperm motility, ALH,LIN,WOB,MAD,STR and hormal morphology rate were 0.080,-0.053,0.174, 0.046,-0.299,-0.221,-0.291,-0.289,-0.297,-0.302,0.176,the differences were not significant(P>0.05).In normal vitality group,correlation coefficient of DFI and total sperm was 0.446, the correlation was statistical significance (P<0.05); and correlation coefficients of DFI and age, sperm concentration, semen volume, sperm motility, PR, VAP, VCL, VSL, ALH, LIN, WOB,MAD, BCF, STR, normal morphology rate were 0.116,0.129,0.337,-0.136,-0.222,-0.198,-0.088,-0.232,0.029,-0.156,-0.210,0.030,-0.178,-0.106,-0.168, correlation analysis showed there were no significant difference (P> 0.05).Conclusions Asthenospermia patients not only show low sperm motility after Semen routine inspection, but also show decreased sperm concentration, decreased total sperm counts, decreased sperm motility characteristic parameters, and increased sperm nuclear DNA fragmentation index; there are a significant correlation between sperm motility parameters and forward motion ratio, which is a detailed evaluation index for sperm motility; sperm nuclear DNA fragmentation index was negatively correlated with sperm motility and sperm velocity in Asthenospermia patients, and it was weakly correlated with other parameters, and this can be used as an independent evaluation indicator of sperm quality. |
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