Application And Study Of Two-dimensional Correlation Spectroscopy In Drug Fast Detection

Currently,drug safety is a big issue around the world and the counterfeit drugs make health under serious threat which interference the normal development of pharmaceutical market.A wide variety of sophisticated means of counterfeit urges the rapid development of analytical techniques.The index of the ability of analytical techniques is comprehensive,which includes rapidity,simplicity,economy and accuracy,anyone of the indicators not meeting the requirements will limit the popularization of technology.Many methods have been developed to identify the counterfeit(s)and adulterant(s),including chemistry,spectroscopy,and chromatographic separation-based methods.Infrared spectroscopy and Raman spectroscopy which can provide rapid,sensitive,non-destructive analysis of samples processing are very suitable for drug rapid detection as an in site,rapid screening tool.In recent years,with the development of spectral data processing technology,for example two-dimensional correlation analysis,the Infrared spectroscopy and Raman spectroscopy have been widely applied in many fields.In this study,an overview of the development and advantages of Infrared spectroscopy,Raman spectroscopy and two-dimensional correlation spectroscopy was given.Besides the application in counterfeit drug detection of the combination of Infrared spectroscopy,Raman spectroscopy and two-dimensional correlation spectroscopy have been introduced.Then three instances were elaborated about two-dimensional correlation spectroscopy combined with spectroscopy applied to the illicit drug detection.Part ?.Overview.Infrared spectroscopy and Raman spectroscopy technology complement each other to provide a rapid,simple,sensitive and non-destructive testing analysis which with a growing application.Two-dimensional correlation spectroscopy can enhance the spectral resolution and solve the existing problems of spectral analysis,i.e.the low resolution and the tough in overlapping peaks analysis.It is common to use two-dimensional correlation spectroscopy in environment,food and medicine analysis.Second derivative pretreated two-dimensional correlation spectroscopy,projection two-dimensional correlation spectroscopy and double two-dimensional correlation spectroscopy improve the quality of original two-dimensional correlation spectroscopy and the application of these new methods provide a kind of new solution towards on-site drug fast detection.Part ?.Two-dimensional correlation infrared spectroscopy applied to the identification of ephedrine and pseudoephedrine illegally adulterated in slimming herbal products.The presence of ephedrine and pseudoephedrine in adulterated slimming herbal products may cause severe harm to patients who continuously take high dose of illicit slimming herbal products,although both of ephedrine and pseudoephedrine promote short-term weight loss.In this study,two-dimensional correlation spectroscopy with second derivative pretreatment was applied to identify trace amounts of ephedrine and pseudoephedrine(0.5-1% to 5% contents)in slimming herbal products.Second derivative pretreatment was carried out on dynamic infrared before two-dimensional correlation spectroscopy to further enhance the spectral resolution and reduce the limit of detection.The detection of ephedrine and pseudoephedrine in simultaneously adulterated SHPs was more challenging compared with the detection of individual adulterants or two completely different chemicals an asynchronous spectral analysis was also applied.In addition,this method was also used to analyze real slimming herbal products in which the adulterants ephedrine and pseudoephedrine were verified successfully by liquid chromatography triple quadrupole mass spectrometry.Part ?.Two-dimensional correlation Raman spectroscopy applied to the drug fast detection.According to the method of characteristic peaks matching in two-dimensional correlation spectroscopy,the conclusion of adulteration can be drawn without complicated sample pretreatment.The spectral resolution was enhanced,however,the peaks from matrix make two-dimensional correlation spectroscopy crowded and complicated,thus it is difficult to identify whether the sample was adulterated.So a simple pretreatment step will usually be imperative before two-dimensional correlation spectroscopy.Two-dimensional correlation spectroscopy combined with thin layer chromatography-surface enhanced Raman scattering(TLC-SERS)maintained the advantage of TLC-SERS and enhanced the spectral resolution which can magnify the minor differences between two similar spectra and finally successfully distinct the two structural analogues.In this study,we use 2DCOS-TLC-SERS to differentiate lovastatin and simvastatin in illegally adulterated lipid-lowering products.The samples were continuous exposure in laser to collect dynamic spectra.The 1D-SERS and SD-SERS of lovastatin and simvastatin are very similar,so it is difficult to discriminate them.However,the two-dimensional correlation spectroscopy can catch the difference of them thus to distinguish them.TLC-SERS combined with two-dimensional correlation spectroscopy can successfully distinguish lovastatin and simvastatin which provide a new strategy in drug fast-screen.In recent years,the trend of drug discovery is high efficiency and low toxicity.So pills usually have lower content active pharmaceutical ingredients(API)and higher content of adjuvant.The spectral information of API will be totally embedded in the spectral of adjuvant which leads to the phenomenon of weak API signal.This will lead to the false positive result in drug fast-screen.Two-dimensional correlation spectroscopy combined with normal Raman spectroscopy successfully discover the characteristic peaks of API covered by peaks of adjuvant.In this study,we choose pills of glipizide and glimepiride as subjects whose spectra were very similar to the spectra of lactose and we choose the laser power intensity change as perturbation to collect dynamic spectra.Raman spectroscopy combined with two-dimensional correlation spectroscopy,projection two-dimensional correlation spectroscopy and double two-dimensional correlation spectroscopy can successfully detect characteristic peaks of API in pill's spectra.This method provided a new perspective in drugs fast-screening.