The Mechanisms Of Regulation And Intervention Of Traditional Chinese Medicine Research Of Ischemic Preconditioning In JAK2/STAT3 Pathway In Rat Myocardial Cells

Objective: Role of JAK2/STAT3 pathway in ischemic preconditioning on myocardial ischemia-reperfusion injury in rats and investigate the protective mechanism of Jiawei Danshen Decoction on myocardial infarction in rats from signal transduction.Methods:The group(IPC+10058-F4 group +c-myc group(JDD+AG490styrene +JAK group(IPC+AG490 styrene-+JAK(JDD+RPM rapamycin Salvia miltiorrhiza group(rapamycin inhibitors of 90 healthy Wister rats,each 250-300g,adaptive feeding a week after 90 rats according to the random number is divided into normal control group(NC group),ischemia reperfusion group(I/R group),ischemic preconditioning group(IPC),Jiawei Danshen Decoction pretreatment group(JDD)group,ischemic preconditioning group +STAT group IPC+RPM flavored drink +group),ischemia preconditioning group inhibitor alpha cyano-3,4-hydroxy)n-benzyl amine group,Jiawei Danshen Decoction inhibitor alpha cyano-(3,4-hydroxy)n-benzyl amine group,ischemic preconditioning group inhibitor 10058-f4.According to the methodology of pharmacological experiment "(Xu sy,Bian RL,Chen Xiu.Experimental method in pharmacology study [M].3 edition.Beijing: People's Medical Publishing House,2001:1179.modeling methods of the production model of myocardial ischemia / reperfusion injury.The real-time monitoring of ECG,ECG ST segment elevation to successful ligation.After the end of 1 rats in each group of each three to Evans-bule,TTC double staining was performed to determine the area of myocardial infarction,myocardial infarction,myocardial necrosis area in ischemic myocardium weight and the weight ratio of said.Then the rats of each three paraffin,paraffin embedding,slicing,xylene dewaxing,conventional HE staining,resin sheet.Under the light microscope with HP10x40 cell line mentality study.The expression of 2 rats each take three Western blot measured the myocardial cell JAK2,STAT3 protein.Determination of 3.PCR gene expression in rat myocardial cells in c-myc groups.The application of SPSS 17 for Windows statistical software test results were analysed statistically.Multiple groups were compared with single factor analysis of variance,P <0.05 was considered statistically significant.Result:1 ECG arc like elevation ligation is successful.Coronary artery ligation in different time after ischemia can cause myocardial cell injury in different degree.I/R group ST to raise about 0.75(a vertical lattice background ECG recorded as 1).5 min ligation Jiaweidanshenyin preconditioning group and IPC group repeated 4 times,10 min reperfusion after ischemia preconditioning,ligation,ST elevation was0.50,down about 33.3%.The 2.IPC group is 11% lower than I/R group infarct area;compared with JDD group and IPC group,3% reduced infarct size.IPC+AG490 group compared with IPC group,infarct area increased by 5%.3.The pathological results: I/R group myocardial fiber variable length,arranged in disorder and fracture,muscle fiber spacing widened,sarcomere shortening,arranged irregularly,interstitial edema,hyperemia or bleeding,inflammatory cell infiltration.IPC group and Jiawei Danshen Decoction pre treatment group,although the myocardial injury but cardiac myofibrils arranged is neat,sarcomere arranged more neatly,occasionally infiltration of inflammatory cells and red blood cell lesions compared with I / R light.Gray 4.I/R group was lower than the other 8 groups,there was significant difference(p<0.05).IPC group,JDD group,JAK2 STAT3 expression compared with I/R group,there is significant difference(P <0.05),and IPC group compared with JDD group,there was no difference in the results(P >0.05).IPC+AG490 group,IPC+RPM group,IPC+10058-F4 group,the gray value of STAT3 was significantly lower than NC group,there was significant difference(P<0.05).In group IPC+AG490,the gray value of JAK2 was lower than that of the other group,there was significant difference(P<0.05),and IPC+RPM group gray value was slightly increased,there was significant difference(P<0.05).,IPC group,JDD group and I/R group,there is significant difference(p<0.05).Results there was no difference between group IPC and group JDD(p>0.05).5 I/R in group myc was low,the high expression of myc in the IPC group,there was significant difference(P<0.05).Group JDD myc gene expression was higher than that of I/R group,there was significant difference(P < 0.01).IPC+RPM group STAT3 was inhibited after myc gene expression was low,there was significant difference(P<0.01).Conclusion:Presence of ischemia reperfusion injury in acute myocardial infarction after reperfusion,ischemic preconditioning can be effective against the injury of ischemic preconditioning on myocardial protection mechanism of JAK/STAT pathway.In the JAK2/STAT3 pathway,JAK2 regulates STAT3,STAT3 regulates c-myc,has a certain effect on the expression of c-myc and STAT3,on the expression of STAT3 in JAK2 also has a feedback effect.They are common in ischemia reperfusion to protect myocardial ischemia injury in rats.Jiawei Danshen Decoction pre treatment on myocardial ischemia reperfusion injury has a protective effect,reduce myocardial ischemia reperfusion injury,reduce the elevation of electrocardiogram ST segment,the morphological changes of myocardial cells,and the mechanism of JAK / STAT pathway related to the intervention,Jiaweidanshenyin preconditioning reasonable through the activation of JAK / STAT pathway,upregulation of c-myc gene expression in the protection of myocardial cell apoptosis.