CD4~+CD25~+Regulatory T Cells And Related Cyrokines、 SOCS1 With Hashimoto’s Thyroiditis

Objective:The aim of study was to explore the immune mechanism of HT,which by estimated the percentage of CD4~+CD25~+Treg,the mRNAlevels of Foxp3 and SOCS1 and serum concentration of TGF-β1and IL-10 in patients with Hashimoto’s thyroiditis(HT) and healthy controls(HCs). Methods:We studied 40 patients with HT and 20 healthy controls in our hospital from November 2014 to August 2015. The CD4~+CD25~+Treg cells in the Peripheral blood mononuclear cell(PBMC) of two group examed by flow cytometry,the concentration of TGF-β1 and IL-10 by ELISA and expression levels of SOCS1 mRNA and Foxp3 mRNA by qRT-PCR. Result:Compared to HCs, HT patients had a significant decrease in peripheral circulating CD4~+CD25~+Treg cells and mRNA levels of Foxp3( P < 0.05). The mRNA levels of SOCS1 were markedly higher in HT patients than HCs( P < 0.05).However,serum concentration of TGF-β1and IL-10 were no significantly difference between HT patients and HCs(p>0.05).Moreover, analysis of Spearman correlation revealed that the ration of Treg and the mRNA levels of Foxp3 and SOCS1 had no correlated with the titers of serum TGAb and TPOAb(p>0.05). Conclusion:Taken together,our data showed that the development of autoimmune process in HT may be associated with CD4~+CD25~+Treg and mRNA levels of Foxp3 and SOCS1.Additionally, there were no markedly difference betweem serum concentration of TGF-β1and IL-10, we speculated the result may be associated with the lower expression of serum content by themselves in HT patients.