Effects Of Cytokine Induced Killer Cells Combined With P38 Inhibitors On Esophageal Carcinoma Cells EC109

BackgroundEsophageal carcinoma(EC)is a malignant tumor with a high incidence and high mortality and the mortality rate ranks the fourth in China.In recent years,the diagnosis and treatment of malignant tumor has a lot of progress,but the prognosis of patients with esophageal carcinoma is still not optimistic,and more than 85% of the patients who diagnosed with EC was already in middle-late,and the 2 year survival rate was less than15%.CIK(cytokine induced killer,CIK)cells have high activity and tumor killing capability with wide spectrum,low toxicity to normal tissues,is currently widely used in clinic.Studies have shown that CIK cells could inhibit the proliferation of EC in vitro and in vivo.As shown in a previous study,p38(38 kda mitogen-activated protein kinase of MAPK)pathway plays an important regulatory role in the process of apoptosis.p38 inhibitors can inhibit EC cell proliferation,and recent studies have shown that p38 inhibitors can prolong the life of T cells,enhance its activity.Therefore,based on the biological function of this dual p38 inhibitors,the study was to investigate the efficacy of CIK cells combined with p38 inhibitors for the treatment of EC cells,which would provide certain experimental basis for the clinical treatment of CIK.ObjectiveTo investigate the effects of CIK cells combined with p38 inhibitors on esophageal carcinoma cell EC109.MethodsHealthy human peripheral blood mononuclear cells were isolated and induced to CIK cells,and flow cytometry instrument was used to detect the immune phenotype.The esophageal carcinoma cell EC109 were divided into 4 groups: blank control group(withoutany treatment),p38 inhibitor group(adding p38 inhibitor),CIK group(adding CIK cells cultivated for 14 days)and combined group(adding p38 inhibitor and CIK cells).Lactate dehydrogenase release method was used to detect the survival rate of esophageal carcinoma cells EC109 in each group.The phase arresting rate and apoptosis rate of esophageal carcinoma cells EC109 in each group was detected by flow cytometry.Western blot was conducted to measure the protein expression of p38,p-p38,FAS and OX40.Results1.In blank control group,p38 inhibitor group,CIK group and the combined group,the survival rate of esophageal carcinoma cells EC109 was 100.0%?75.0%?50.0% and25.0% respectively,the survival rate of esophageal carcinoma cells EC109 in late three groups were significantly lower than those in blank control group(P<0.05),while they were significantly lower in combined group compared with those in p38 inhibitor group,CIK group(P<0.05)..2.The G1 phase arresting rate of esophageal carcinoma cells EC109 was 31.46%?42.04%?50.16% and 72.02%,phase arresting rate of esophageal carcinoma cells EC109 in late three groups were significantly higher than those in blank control group(P<0.05),while they were significantly higher in combined group compared with those in p38 inhibitor group,CIK group(P<0.05).3.The apoptosis rate of esophageal carcinoma cells EC109 was 7.15%?19.31%?42.15% and 67.17% respectively.The apoptosis rate of esophageal carcinoma cells EC109 in late three groups were significantly higher than those in blank control group(P<0.05),while they were significantly higher in combined group compared with those in p38 inhibitor group,CIK group(P<0.05).4.Fas,OX40 expression were increased in late three groups(P<0.05).ConclusionCombination of CIK with p38 inhibitors has powerful effect of killing activity on esophageal carcinoma EC109 cells.