Clinical And Prognostic Research On DNA Content Of Esophageal Squamous Cell Carcinoma With Flow Cytometry (FCM)

Background: Esophageal carcinoma is one of the commonly malignant tumor. Our country belongs to the high incident nation with the scope of the world. It is seriously threat the safety of people's life. Recently years the patients prognosis was not well though more and more research had be done. 5 year survival rate was only 20%. The change of esophageal squamous cellular DNA content was one of the important biological characters in malignant tumor .We want to detect esophageal squamous cellular DNA content and cellular proliferation activity by Flow cytometry (FCM).To analyze DNA content and cellular proliferation activity and find out their clinical significance in tumor's incidence, development, invasion and metastasis. They help to evaluate diagnosis, treatment, degree of malignant and prognosis of esophageal squamous by combined with clinical information of patients. Objective: We want to detect esophageal squamous paraffin-embeded pathological material cellular DNA content and cellular proliferation activity by Flow cytometry (FCM). To analyze the relationship between DNA content with their clinical and prognostic significance. Directing therapy, improving the patients survival rate.Methods: 135 cases of patients were selected randomly from those who underment surgery from 1998.1 to 1999.12. Choosing paraffin-embeded pathological material of esophageal carcinoma .Using Flow cytometry (FCM) detect DNA index (DI), DNA ploid, aneuploidy, S-phase fraction (SPF). Analyzing the consequence with their clinical feature, then comes to the conclusion.Results: The 1,3,5 year of survival rate are 79.26%, 53.67%, 32.70% in 135 cases of patients. Hetero-ploidy incidence of esophageal squamous was 66.67 %.DNA ploid had no correlated with patient's age, sex, tumor position. It had correlated with tumor length, invasion depth of the tumor ,lymph node metastasis, tumor differentiation degree and pathological grade. The SPF had no correlated with patient's sex, tumor position and tumor length. It had correlated with age, invasion depth of the tumor, lymph node metastasis, tumor differentiation degree and pathological grade. There was significant different in prognosis between the group of diploid and the the group of hetero-ploidy. Conclusion: To detect esophageal squamous paraffin-embeded pathological material DNA content and cellular proliferation activity by flow cytometry(FCM). To analyze the relationship between DNA content and tumor pathological, clinical features. To judge prognosis and direct therapy, improving the patient's survival rate. We think simple TNM stage can not be the enough evidence of therapy and judging prognosis. Accordingly, detect cellular DNA content and cellular proliferation activity by Flow cytometry of esophageal carcinoma is prospective.