Effect Of Body Weight Support Treadmill Training Combined With Methyprednisolone On Hindlimb Motor Function In Injured Spinal Cord Of Rats

ObjectiveThrough the establishment of incomplete spinal cord injury model in rats to simulate the clinical patients with spinal cord injury. To explore the effects of body weight support treadmill training combined with methylprednisolone on motor function and expression of brain-derived neurotrophic factor and tyrosine kinase receptor B in rats with spinal cord injury, provide theoretical basis for clinical treatment of spinal cord injury. Methods(1)Groups:Clean level forty-eight adult male SD rats were numbered and randomly divided into damage control group(SCI), body weight support treadmill training group(BWSTT), methylprednisolone group(MP), body weight support treadmill training combined with high-dose methylprednisolone group(BWSTT+MP), Each group of 12.(2)Modles: incomplete spinal cord injury rats model of each group were established by using modified Allen's method at T10 level of spinal cord.(3)Process:SCI group was no processing after injury, BWSTT group was trained by body weight support treadmill training for 4 weeks, MP group was treated by high dose methylprednisolone and combined group was treated by high dose methylprednisolone and trained by body weight support treadmill training. Motor function of every group was assessed by using BBB and Modified Tarlov scale before injury and at the 1st, 2nd, 3rd, 4th, 5th week post injury. The expression level of BDNF and Trk B in spinal cords were detected by immunohistochemical method at a week after injury and the end of the training. Results(1)Behavioral assessment results:? 5 w motor function of each group after injury rating score significantly higher than 1 w( P < 0.01);? Since the 3rd week after injury,scores of BBB and Tarlov in more than three sets of group were higher than that in damage control group;Combined group were higher than that in BWSTT and MP group(P < 0.05).(2)Immunohistochemical results:? One week after injury, positive cells of BDNF and Trk B of four groups are increased,but four groups was no statistical significance;? At the end of the training,compared with SCI group,the positive cells of BDNF and Trk B in combined group,BWSTT group and MP group was significantly higher,and combined group is obviously higher than BWSTT group and MP group(P < 0.05). Conclusion(1)Body weight support treadmill training and Methylprednisolone can promote the recovery of motor function and the expression of BDNF and Trk B in spinal cord injury rats.(2)Body weight support treadmill training combined with methyprednisolone can more effectively promote the recovery of motor function and the expression level of BDNF and Trk B of SCI rats than pure body weight support treadmill training and methyprednisolone.