The Role And Potential Mechanisms Of TCONS₀0023979 In Prostate Cancer: A Preliminary Study

Objectives: To gain the expression profile variation of long non-coding RNAs?lnc RNAs?and m RNAs between prostate cancer?PCa?tissues and corresponding adjacent non-tumor tissues;To verify the expression of the key lnc RNA molecule TCONS₀0023979?lnc RNA ID?and nearby gene PML,and further investigate the potential regulatory mechanism of TCONS₀0023979;To explore the role of TCONS₀0023979 in the proliferation and invasion of prostate cancer cell lines.Methods: 1.High throughput lnc RNA and m RNA microarrays were used to compare the lnc RNA and m RNA expression profile difference between prostate cancer tissues and corresponding adjacent non-tumor tissues.2.Screening important lnc RNA which is relative to the progression of prostate cancer through strategies such as change fold,neighboring genes analysis and relevant literatures.3.The expression levels of TCONS₀0023979 and PML were verified by using q RT-PCR in 13 PCa tissues and corresponding adjacent non-tumor tissues.4.Specifical si RNA for suppressing TCONS₀0023979 was employed to downregulate TCONS₀0023979 expression in human PCa cell lines DU145 and LNCa P.5.Bioinformatics analysis were applied to forecast the target gene of TCONS₀0023979,and the expression of the target gene was verified by RT-PCR and western blots after knockdowning TCONS₀0023979.6.MTT assays,plate clone formation assays and transwell assay were employed for investigating the proliferative and invasive properties of each PCa cell lines after suppressing TCONS₀0023979.Results: 1.The results from high throughput lnc RNA and m RNA microarrays suggested that many lnc RNA and m RNA molecules were regarded as ones with differential expression,and then 9 m RNAs and 13 lnc RNAs were selected by some strategies.2.We found that TCONS₀0023979 may play the key role in PCa after a series of analyses,and q RT-PCR results showed that TCONS₀0023979 was frequently down-regulated in prostate cancer tissues when compared to corresponding adjacent non-tumor tissues which keep the same with the results of gene microarrays.3.Bioinformatics predicted that PML,which is one of the anti-oncogenes of PCa and is in the downstream of TCONS₀0023979,may be a target gene for TCONS₀0023979.The expression of the target gene was found to be down-regulated by RT-PCR and western blots after suppressing TCONS₀0023979.4.The proliferative and invasive properties of PCa cell lines got enhanced after the expression of TCONS₀0023979 was suppressed.Conclusions: We reported a group of prostate cancer related lnc RNAs and m RNAs for the first time by high-throughput screening of lnc RNAs microarrays.The long non-coding RNA TCONS₀0023979 may regulate actively its target gene PML.TCONS₀0023979 is one of the key lnc RNA molecule which can suppress the proliferative and invasive properties of PCa cell lines by modulating PML.