| In recent years, more and more patients died of cancer. The main reason for this phenomenon is that it is not easy to detect the tumor at the early stage and difficult to cure when it is detected at the late stage. The treatment of cancer is still a huge challenge in medical field. Therefore, early detection and treatment of tumors is very important in the clinical applications. Conventional imaging techniques is the mainly detection means for tumor currently, such as CT, ultrasound imaging, MRI and X-rays, etc. However, the problem is that only when the organs have obvious lesions can the tumor be detected. With the development of science and technology, molecular imaging appeared. The appearance of molecular imaging can compensate for the lack of traditional imaging, it can detect the tumor earlier. The widely used technology, multi-modality imaging technology can resolve the defects of single imaging modality.The core issue of multi-modality imaging technology is manufacturing multimodal probes which can be used in varieties of imaging modalities. An bimodal nano-probe is manufactured in this paper which can be used for fluorescence imaging and magnetic resonance imaging, it can not only solve the low sensitivity problem of fluorescence imaging and low resolution problem of MRI but also can improve the accuracy of imaging. The preparation of the magneto-optical nanoprobes can be divided into the following four steps:(1): Select Cy5.5 as the fluorescent markers which are activated by the NHS and EDC.(2): Select the iron oxide as the magnetic nanoparticles and make it to be water-soluble by using CTAB.(3): Use the silica to clad the iron oxide and make the Cy5.5 to adsorb in the surface.(4): Use the PEI to cover the outermost and react with NHS in Mal-PEG-NHS. The group exposed on surface of magneto-optical nanoprobes which is composed successfully finally is Mal. The group can crosslink antibody for the post-nanometer and make it impossible to compose the active targeting nano-probe. The magneto-optical nanoprobes which is composed successfully in this paper is called CFN-NPs.In order to explain the physical and chemical properties of the new magneto-optical nanoprobe which is made in this paper as well as whether it can be absorbed and used by the cells, following experiments are made. First of all, it is confirmed that there are fluorescence signals and magnetic signals, by selecting the vitro characterization, the particle size of it is at the nano level; it has good dispersion and can be absorbed by the cells in vivo. Secondly, it is verified that it has good biological compatibility, low cytotoxicity and it can be absorbed by the cells by conducting a series of cell experiments. Finally, significant fluorescence signal and a magnetic signal can be observed and it can be targeted to the tumor when it is injected in vivo by conducting vivo fluorescence imaging and magnetic resonance imaging experiments.Experiments show that the synthetic magneto-optical nanoprobes in this paper has a lot of advantages, such as moderate condition requirement, uniform particle size, good and stable dispersion. It can be swallowed by cells, has significant fluorescence signal and magnetic signal in vivo experiments and has the passive tumor targeting ability. It is expected to be used to detect the preclinical tumor in the future. |
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