COMMD7 Gene Promotes HepG2 Proliferation And Migration By Activiating The PI3K/AKT Signal Pathway

The purpose and significance:Hepatocellular carcinoma(HCC)is the fifth most common cancer worldwide,with at least 1 million new cases each year.Even if liver transplantation remains the ideal treatment,hepatic resection remains the only curative treatment for HCC.Considering the early experience of liver resection for HCC in the 1980 s,results were discouraging,with a mortality rate in the range of 10% and a considerable morbidity.Improvements in patient selection,early diagnosis,preoperative and postoperative management,surgical technique and development of new technologies have allowed to obtain a lower mortality and morbidity,achieving 0% in certain high-volume centers.The development of laparoscopic and robotic surgery,associated with the application of new technologies in patient care and progress in the medical treatment of HCC,represent a modern era challenge to optimize the management of HCC with the objective of improving overall and disease-free survival.The aim of this article is to describe all innovations in the surgical treatment of HCC.Methods:The specific si RNA(small interference RNA)was designed for COMMD7 gene,si RNA transfected HepG2 cells was set as Si-HepG2 group,and the other groups were also set up: PTENinhibitor treating group(Si+Bp V-HepG2),controlgroup(HepG2),the emptyve ctorgroup(HepG2 was infectedemptyvector,N-HepG2group).qRT-PCRwasperformedtoevalu atetheexpressionof COMMD7 and PTEN,Westernblot was performedto test theexpressionof COMMD7,PTEN,p-AKT,and AKT.MSP method wasperformedto detectmethylationlevel,CCK8 was performed totest cellproliferationability;Transwellwasperformedtodetect the inva sion of cells.Results:The results of RT-PCR showed that the expression of COMMD7 gene in Si-HepG2 group was lower(0.101 times)than control group and N-HepG2 group,the expression of PTEN gene was higher(2.841 times)than control group and N-HepG2 group,these all were statistical differences(P<0.05).The results of Western Blot showed that the expression of COMMD7 gene reduced in Si-HepG2 group,the expression of PTEN increased,the expression of the following p-AKT was inhibited;the expression of PTEN in Si+Bpv-HepG2 group was inhibited obviously,the expression of the following p-AKT increased.MSP was performed to test the methylation level of PTEN gene: Si-HepG2 group was more obviously methylated than control group and N-HepG2 group.The results of CCK8 showed that the proliferation in Si-HepG2 group was decreased comparing control group,N-HepG2 group and Si+Bp V-HepG2 group.The results of Transwell showed that the numbers of cell permeating septum in Si-HepG2 group,control group,N-HepG2 group and PTEN group were 17.4±2.7,41.6±4.5,36.2±3.2,47.6±1.8,respective,with a significant difference among the 4 groups(P<0.05).Conclusions:SiRNA interference decreased the expression of COMMD7 induced the demethylation of PTEN gene in HepG2 and increased the expression of PTEN gene to inhibit PI3K/AKT signaling pathway thus the proliferation migration and invasion ability of HepG2 cells were decreased.