Study On The Mechanism Of HBX Activating MicroRNA-155 Inhibiting The Expression Of PTEN And Promoting The Malign-ant Transformation Of Liver Cancer

Objective: The relationship between HBX,miRNA-155 and PTEN/ PI3K-AKT was observed through the detection of clinical specimens and cell experiments,to explore the effect of HBX activation of miRNA-155 on the PTEN/ PI3K-AKT pathway and to study the molecular regulatory mechanism of HBX on liver cancer.Methods: The study collected the data of patients who underwent hepatectomy and histopathologically confirmed HCC and the serum,the cancer and adjacent tissues of the patients,and 50 normal people who were negative for HBV.The clinical data were retrospectively analyzed.Cell experiment filtered eventually choose SMCC-7721 and HepG2 two cell lines,three groups were performed between groups in western blot,transwell Chambers experiment,scratch test,CCK8 cell proliferation experiment,EdU cell proliferation experiment and experimental testing,each experiment is repeated three times,statistical results between groups.Results: The amount of miRNA-155 liver cancer patients express HBV positive than negative for HBV patients and the control group,patients with HBV positive ? and ? mi RNA-155 expression quantity higher than ? and ? stage patients.Western blot results showed that the expression levels of PTEN and E-cadherin in SMCC-7721 inhibitor group were higher than those in NC,while the expression levels of AKT N-cadherin CyclinD1 and CyclinA1+A2 were opposite.The expressions of PTEN and e-cadherin in the HepG2 mimics group were lower than those in the mimics NC,while the expressions of AKT,N-cadherin,CyclinD1 and CyclinA1+A2 were opposite.Results of Transwell chamber experiment showed that the number of transplanted cells in smcc-7721 inhibitor group was significantly lower than that in inhibitor NC.The number of cells migrated in the mimics group of HepG2 was higher than that in the mimics NC group.Results of CCK8 showed that cell proliferation rate of SMCC-7721 inhibitor group was significantly lower than that of inhibitor NC group.The cell proliferation rate in the HepG2 mimics group was significantly higher than that in the mimics NC group.Results of EDU proliferation assay showed that the positive rate of EDU in smcc-7721 inhibitor group was significantly lower than that in inhibitor NC group.The positive rate of EDU in HepG2 mimics group was higher than that in mimics NC group.The results of Transwell chamber experiment showed that the number of migrated cells in smcc-7721 HBX group and HepG2 HBX group was higher than that in the NC group.The results of scratch test showed that the cell migration rate of smcc-7721 HBX group and HepG2 HBX group was lower than that of the NC group.CCK8 results showed that the proliferation rate of smcc-7721 hbx group and HepG2 HBX group was significantly lower than that of the NC group.The results of EDU proliferation experiment showed that the positive rate of EDU in smcc-7721 hbx group and HepG2 HBX group was significantly higher than that in the NC group.Western blot results showed that the expression levels of E-cadherin in the SMCC-7721 HBX and HepG2 HBX SiRNA groups were higher than those in the NC group,while the expression levels of N-cadherin,CyclinD1 and CyclinA1+A2 were opposite.The results of Transwell cell assay showed that the number of HBX and HepG2 HBX SiRNA transfected cells in SMCC-7721 and HepG2 HBX SiRNA groups was lower than that in the NC group.The results of scratch test showed that the cell migration rate of SMCC-7721 HBX and HepG2 HBX SiRNA group was lower than that of the NC group.CCK8 results showed that the proliferation rate of SMCC-7721 HBX and HepG2 HBX SiRNA cells was significantly lower than that of the NC group.The results of EDU proliferation assay showed that the positive rate of EDU in SMCC-7721 HBX and HepG2 HBX SiRNA group was significantly lower than that in the control group.Conclusion: The relative expression level of microrna-155 in patients is closely related to positive hepatitis B.miRNA-155 promotes cell invasion and proliferation by inhibiting the expression of PTEN.Over-expression of HBX can promote the expression of miRNA-155,thus promoting cell invasion and proliferation.Knockdown of miRNA-155 can inhibit cell invasion and proliferation.HBX can activate miRNA-155 to inhibit PTEN and promote the malignant transformation of liver cancer.