Chemical Constituents Of The Lllicium Jiadifengpi B.N.Chang And Endophytic Fungi From Rabdosia Rubescens And Their Anti-tumor Secodary Metabolites

BACKGROUND:This dissertation is composed of two chapters. The first part is the chemical composition of illicium jiadifegnpi, the second part is endophy fungi from rabdosia rubescens and their anti-tumor secondary metabolites.The study was supported by the National Natural Science Foundation of China (No:81172961). The dried root bark and stem bark of illicium jiadifengpi B.N.Chang, which belongs to Illiciaceae is a folk Chinese herbal drug.It mainly in southern Anhui province, southeastern Hubei, Guangxi and other places,which is our unique plant.Contains in (zhejiang flora), (zhejiang folk), its root bark and bark with treatment functions of the effect of pain and injuries, have small poison.It often known as maple skin falsify, 1982 reported illicium was found to be a new species of plants, because in recent years, as the social ages, the study found that false to maple in the skin and a half times the terpene lactone class composition with promote neurite growth of pharmacological activity, received extensive attention of many scholars.OBJECTIVE:This article through to the false fruit maple skin was studied by means of chemical composition, in order to clear the false maple skin against hepatitis b virus active ingredients and antibacterial active ingredients.The new structure of antiviral and antibacterial discovery of active compounds and maple to fake leather plant resources protection and development of the application has a certain significance.To further clarify the false maple skin toxicity component, using the method of processing to remove or reduce their toxicity, so it can be to maple skin substitution materials, ease of maple skin resources nervous, at the same time, a certain basis for the development of new drugs.The second part:Rabdosia rubescens is Labiatae rabdosia of broken rice fork variant.Which have very strong cold resistance, to positive Yin plant resistance.Then mainly distributed in the south of the Yangtze river and Yellow River basin and the region, leading producer in jiyuan, henan taihang mountain area, dong ling grass taste bitter, small cold, anti-inflammatory analgesic, clear heat, nourish and anti-tumor effect and so on.METHODS:Based on the normal phase silica gel column, MCI, macroporous adsorption resin column SephadexLH-20, the preparation of liquid chromatography for separation and purification, and using modern spectrum techniques such as: Ultraviolet Spectroscopy (UV) and Infrared Spectrometry (IR), Mass Spectrometry (MS), nuclear magnetic resonance spectroscopy (13C-NMR) carbon and hydrogen nuclear magnetic resonance spectroscopy ('H-NMR) on the structures of the isolated compounds.Dilution method using twelve sesquiterpene compounds isolated antibacterial activity.Second, there are many different kinds of plant endophytic fungi in the body and widely distributed? In recent years, from the plant endophytic fungi secondary metabolites of points in the novel structure and has unique physiological activity compounds, the paper design to dong ling grass as the research object, from dong ling grass in the secondary metabolites of endophytic fungi isolated from compounds with antitumor activity.RESULTS:(1) From the false in maple skin identified 16 compounds, among them 8 compounds isolated from false maple skin for the first time identified, respectively Icariside E4,1 S-miwaneone,1 R-miwaneone,6-deoxy-hydroxyneoanisatin, anislactone B, anislactone A, (1?,4?,6?)-gorgonane-1,4,11-triol, (1S,2S,4S, 6R)-4-Isopropenyl-1-methyl-7-oxabicyclo-[4,1,0]-heptan-2-ol.Separate identification of compounds in 12 sesquiterpenoids, a single terpenoids,1 lignans, and five other ingredients.Studies have shown that false fruit maple skin contains the characteristics of the chemical composition is contains a lot of times and half terpene lactone class composition.Eight sesquiterpenoids there were no inhibition on escherichia coli and staphylococcus aureus.(2) Isolated from Rabdosia rubescens got 18 strains of endophytic fungi, which is dominant population of endophytic fungi alternaria,50% of the total.Some endophytic fungi have antitumor activity.