| With the rapid development of nuclear and radiation technology and their applications in human life, people are exposed to radiation increasingly. Hematopoietic system is extremely sensitive to radiation. Radiation cause damage to hematopoietic stem cells (HSC), as well as the hematopoietic microenvironment in which HSC resided. We selected Bushen Kangshuai tablet (BSKS) among several Chinese medicine, hoping that BSKS could improve hematopoietic reconstitution in mice after radiation-induced damage.As is known to all, Bone marrow mesenchymal stem cells (MSC) are important components of bone marrow hematopoietic microenvironment, and it has the potential of multi-directional differentiation. First, we isolated MSC by the whole bone marrow adherence method, and then induced these cells into osteoblasts (OBs) that can be identified by alkaline phosphatase (ALP) activity detection and alizarin red staining. These results proved that the cells obtained by the whole bone marrow adherence method were actually bone marrow MSC.To investigate the effects of BSKS on mesenchymal stem cells (MSC) and hematopoietic stem cells (HSC), and on regulating the supportive function of MSC to HSC. Mice were treated with 0.75 g/kg,1.5 g/kg,3.0 g/kg body weight BSKS suspension orally before and after 6.0 Gy 137Cs-? ray irradiated. And then CFU-S, CFU-F, CFU-GM and 2D CFU-GM were detected. It was found that BSKS could promote colony formation of CFU-S and CFU-GM, indicating that BSKS could enhance the proliferation of HSC; BSKS could promote colony formation of CFU-F, indicating that BSKS could enhance the proliferation of MSC; BSKS could promote colony formation of 2D CFU-GM, indicating that the MSC which were treated with BSKS could improve the proliferation of HSC. BSKS showed significant protective effect at the dose of 1.5 g/kg. Next, we set 1.5 g/kg dose group and normal saline group as the treated croup and the control group respectively, and mice of both groups were treated with 6.0 Gy 137Cs-? ray radiation, and then we examined the pathological morphology of the bone marrow. Results showed that BSKS could promote the proliferation of bone marrow stromal cells and hematopoietic cells significantly.To observe the influence of BSKS on protection of bone marrow blood cell genetic radiation damage. Bone marrow polychromatic erythrocyte (PCE) micronucleus rate of mice after 24 hours (treated with 2.0 Gy irradiation) and 7 days (treated with 6.0 Gy irradiation) were detected respectively. We found that the rate of the agent group (1.5 g/kg BSKS) significantly decreased compared with that of the irradiated control group.To study the effect of BSKS on peripheral blood of mice with radiation injury, mice were exposed to sub-lethal dose of whole body radiation. The white blood cells (WBC), red blood cells (RBC) and platelets (PLT) in peripheral blood were detected after 7days. BSKS showed some promoting effects on WBC, RBC and PLT.We used MSC and bone marrow cells co-transplant method to study the effect of MSC treated with BSKS on the biological effects of radiation injured mice. The survival of mice within 30 days were observed after the transplantation. Results showed that the survival rate of mice in the treated group was higher than that in the control group.In order to explore the molecular mechanism of BSKS on hematopoietic reconstitution, a preliminary research on the expression levels of Jaggedl in MSC were detected, which is one of hematopoietic growth factors expressed on MSC. The result showed that Jaggedl manifested positive expression in both of the control and the treated group, while there was no significant difference between the two groups. |
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