Correlation Analysis Of Karyotype And Gene Mutation With Prognosis Of Acute Myelocytic Leukemia

ObjectiveTo explore the value of karyotype and gene mutation in the diagnosis and prognosis of acute myelocytic leukemia (AML).MethodsChromosome analysis of newly diagnosed AML patients were performed with G-banding techniques. Internal tandem duplication(ITD) of 14th and 15th exon in FLT3 gene were tested by PCR and agarose gel electrophoresis. PCR combined with Sanger sequencing technique were used to detect mutations of c-KIT/D816 gene?12th exon of NPM1 gene and CEBPA gene. The overall survival times were calculated by follow-up visit.Results73 (52.9%) chromosomal aberrations were detected. The most common types were t(15;17), t(8;21) and +8. There were statistically significant differences in age and white blood cell counts with initial diagnosis (P<0.05). The median time of follow-up visit in 110 patients was 8 months. The 3-year survival rates of the low risk group, intermediate risk group and high risk guoup were 84.0%,45.3% and 21.0% respectively (P<0.05).51 chromosomal aberrations were detected in the 109 cases of genen mutation analysis. The mutations of FLT3 gene were 17 cases, c-KIT gene were 3 cases, NPM1 gene were 9 cases, and CEBPA gene were 6 cases. The median survival time of negative and positive group of FLT3 gene mutation in normal karyotype patients were 22.5 and 5.2 months respectively. The 3 years of survival rates were 38.1% of negative FLT3 mutation groups and 22.2% of positive FLT3 mutation group.ConclusionThe various subsets of AML had different chromosomal aberrations, which could be applied for the diagnosis and prognosis monitoring of AML. The mutations of FLT3 belongs to the adverse factors in the prognosis of AML, that affect the NPM1 and CEBPA prognosis of AML.