Research Progress Of Total Saponins Of Ziziphi Spinosae Semen On Extraction, Purification And Biotransformation Of Jujuboside A

Objective:Research has sedative effect of Semen extraction and purification of total saponins, optimizing optimum conditions; in order to enhance its efficacy research Jujuboside A biotransformation, guide the development of efficient and sedative drugs.Method:Single factor experiment, orthogonal design to Jujuboside A major investigation index, the extraction process of research; to investigate Semen total saponins as the main indicator of macroporous resin purification process were studied; by acid hydrolysis, EM fermentation, stomach contents outside the object metabolism, intestinal flora in vitro metabolism experiments Jujuboside a biotransformation using HPLC-ELSD and HPLC-MS to infer the structure of transformation products, and by pharmacodynamic experiments comparison of total saponins transformation products sedative effect.Result:A preferred process for the extraction and purification: herbs plus 8 times the amount of 70% ethanol was heated to reflux and extracted three times, each time 1.5h, liquid filtration, merger, centrifugation, vacuum dryer set were concentrated under reduced pressure until no alcohol taste, diluted with water to 0.2g/ml, select D-101 macroporous resin, wet packed column, press herbs 1.5 g/g resin sample volume, the flow rate of 1.0 BV·h-1 in the sample; be alley after sufficient adsorption Jen total saponins, standing 1 h, first eluting with distilled water(2 BV·h-1,3 BV), then 30% ethanol(2 BV·h-1,3 BV) and eluted, and finally with 70% ethanol(2 BV·h-1,4 BV) eluted with 70% ethanol eluent were collected. Eluent decompression recovery ethanol, and concentrated and dried to obtain the total Semen saponin fraction, purity of the components Jujuboside A reached 1.97%, Semen total saponins purity of 82.9%. In vitro metabolism of intestinal flora can be completely converted to Jujuboside A secondary glycosides Jujuboside B, pharmacodynamic results by comparing total saponins in Semen conversion product has a better sedative effect.Conclusion:Preferred Semen extraction and purification of total saponins process is stable, the intestinal flora in vitro conversion can be converted to Jujuboside A secondary glycosides Jujuboside B, and the latter sedative better, in Jujuboside A It remains to be further studied acting the way the final form of the body to absorb and Jujuboside B's.