| Background:Re-endothelialization of the stent helps prevent restenosis after PCI. DES reduced restenosis, but inhibited the proliferation and migration of smooth muscle, delaying the re-endothelialization of the stent.Endothelialization after stent implantation not only included the proliferation and migration of endothelial cells but also included the endothelial progenitor cells (EPCs) mobilization, migration, homing and differentiation methods. Original host endothelial cell proliferation stent adjacent to stent migration, gradually covering the stent is an important way of endometrial repair. Recent studies have shown that statins in addition to having the lipid-lowering effects, but also can promote EPCs mobilization, differentiation, proliferation, migration and homing. The purpose of this study wasObjective:To detecting the reendothelization relevant active substance such as VEGF, eNOS, MMP-9 of the rabbit model after given rosuvastatin 4 weeks and the expression of the antigen RNA about EC and EPCs and reflected by OCT scanning carriage re-endothelialization level to study rosuvastatin on endothelial progenitor cells.through all this to explore the impact of the rosuvastatin on endothelialization after stent implation.Subjects and methods20 New Zealand white rabbits given high fat diet (1% cholesterol+5% lard) and underwent balloon injury seven days later, eight weeks after abdominal aorta, according to the experimental scheme selection 16 rabbits with significant abdominal aortic plaque, then implanted the stent.4 rabbits died within 72 hours after stent planted, the final 12 stent implantation rabbits were randomly divided into control group and rosuvastatin group each group contains 6 rabbits, the model group was given normal saline while rosuvastatin group given rosuvastatin (1mg/kg/d) orally four weeks later, the measured serum MMP9, eNOS, VEGF, qPCR detection of specific of EPCs, EC cell antigen levels, combined with angiography before being killed in the OCT observation stent intimal coverage, take-containing sample processing vessel segments after the stent 4% paraformaldehyde soaked and marked to send Zhongshan hospital of Fudan University, hard plastic after embedding, with a hard tissue slicer, select each stent cut 2 HE staining, Leica image analyzer and a micro-computer plus Scion image NIKON microscope image analysis system vascular stent neointimal slice thickness, surfaceResult1. TG rosuvastatin group and model group (2.02±0.12VS2.59±0.17, P <0.05), rosuvastatin significantly reduced; TC rosuvastatin group and model group (20.51±1.61VS24.92±1.69, P<0.05), rosuvastatin significantly reduced; LDL rosuvastatin group and model group (9.17± 0.74VS11.73±1.42, P<0.05), rosuvastatin significantly reduced2.The result of ELISA:VEGF of the rosuvastatin group compared with the model group (140.1500±48.8489 than 60.6567±15.3222, P<0.05), rosuvastatin group significantly higher; MMP9 of rosuvastatin group compared with the model group (114.1667±32.1603 than 28.3350±16.1084, P<0.05), rosuvastatin group significantly higher; eNOS of the rosuvastatin group compared with the model group (0.3416±0.1401 than 0.0282±0.0131, P<0.05), rosuvastatin group significantly higher than the model group.3.RT-PCR method to detect the expresstion of antigen levels about vessel segment containing the stent:CD34 of the rosuvastatin group compared with the model group (1.6755±0.1049 than 1.0000±0.1189, P<0.05),the expression of the rosuvastatin group significantly increased; VWF of the rosuvastatin group compared with the model group (3.9379±0.6229 than 1.0000±0.0931, P<0.05), the rosuvastatin group expressed better than the model group; VEGFR-2 of the rosuvastatin group compared with the model group compared (8.4210±1.5791 than 1.0000±0.2004, P<0.05), the expression of the rosuvastatin group significantly increased.4.Pathological examination of the two groups by HE:neointimal thickness, the rosuvastatin group compared with the model group (900085±84830 than 395865±154191, P<0.05); neointimal area:the rosuvastatin group compared with the model group (69.11±9.82 than 35.69±15.28, P<0.05);the proliferation of area/bureaucratic area:the rosuvastatin group compared with model group (0.262742±0.045343 ratio of 0.164520±0.054154, P <0.05)Conclusion:1.Rosuvastatin can improve the local drug-eluting stent endothelialization related factors, improve endothelial-related gene expression2. Rosuvastatin can significantly promote the proliferation of drug-eluting stents within the intima thus contributing to drug-eluting stents in the level of re-endothelialization... |
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