| Objective:Analysing the expression and significance of long noncoding RNA (lncRNA) in tumor to grasp the hot spot of lncRNA. Then make a further analysis of lncRNA in order to find out a potential lncRNA associated with prostate cancer. To verify the expression of CCAT1 in prostate cancer tissue by qRT-PCR. Then CCAT1 inhibitor was upregulated in the two androgen-independent prostate cancer (AIPC) cell lines DU145 and PC3, to observe the the changes of proliferation and apoptosis of prostate cancer cells.Methods:1.The articles were searched in the PubMed data base and analysed in the MSKCC data base 2.The CCAT1 expression profiles in prostate cancer tissues and prostate tissues were measured by qRT-PCR.2.Effects of CCAT1 siRNA were evaluated by MTT assay, flow cytometry assay, adhesion assay, respectively.Results:The analysis by MSKCC showed that CCAT1 was one of the hot spots of lncRNA, and showed that CCAT1 levels increased in prostate cancer tissues compared with in prostate tissues, and increased in metastatic prostate cancer tissues compared with in prostate cancer tissues. Real-time quantitative PCR was verified the CCAT1 expression profiles. MTT assay showed that upregulated of CCAT1 siRNA can inhibit the proliferation of prostate cancer cells DU145 and PC3.Flowcytometry indicated that cell apoptosis were significantly higher in CCAT1 siRNA up-regulation group than that in the negative control (NC siRNA) group. All the results of above experiments had statistical significance (P< 0.05).Conclusion:1.CCAT1 is up-regulated expression in CRPC and higher expressed in metastatic prostate cancer.2. CCAT1 significantly suppressed the apoptosis and enhanced their proliferation of CRCP cells. |
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