Expression And Clinical Significance Of Long Non-Coding RNA CCAT1 And H19 In Colorectal Cancer And Its Precancerous Lesions

OBJECTIVE: Colorectal cancer is one of the common malignant tumors in China and even in the world.In this study,Quantitative Real-time PCR was used to detect the expression of long-chain non-coding RNAs CCAT1 and H19 in colorectal cancer,adjacent to cancer and precancerous lesions,and normal colonic mucosal cell lines and colon cancer cell lines.The expression of RNA in the evolution of colorectal tumors(normal tissue,precancerous,cancer),combined with clinical pathological parameters,and explore the role of long-chain non-coding RNA CCAT1,H19 in the development and progression of colorectal cancer and its molecules mechanism.Methods 1.Collecting endoscopic biopsy specimens from The People's Hospital of Inner Mongolia Medical University and tissue specimens undergoing radical resection of colorectal cancer in our hospital,and detecting the expression of long-chain non-coding RNAs CCAT1 and H19 in the above specimens by Quantitative Real-time PCR.Analyzing the correlation between expression level and clinical pathological parameters of patients;2.Colonic mucosal epithelial immortalized cell line NCM460 and human colon cancer cell lines HT29 and HCT116 were cultured in vitro,and the expression of long-chain non-coding RNA CCAT1 was detected by Quantitative Real-time PCR.The long-chain non-coding RNA CCAT1 was silenced by siRNA technique and its effect on colon cancer cell proliferation was detected and observed by IncuCyte S3 and discussing the mechanism to promote colon cancer proliferation.Results 1.The expression of Long non-coding RNA CCAT1 in colorectal cancer tissues,precancerous lesions and normal tissues showed a decreasing trend,and the difference was statistically significant(P<0.05).Among them,the expression of colon cancer tissue 0.00881(0.00001,0.06112)was significantly higher than precancerous lesions 0.002491(0.00002,0.00625)(P=0.0491)and adjacent normal tissues 0.00050(0.00001,0.00486)(P=0.000003);the expression of precancerous in the lesion was significantly higher than that in the adjacent normal tissues(P=0.000001),and the difference was statistically significant(P<0.05).2.The expression level of long-chain non-coding RNA H19 in precancerous lesions was 0.0088910(0.00015,0.05466),which was significantly higher than that in colon cancer tissues,0.00119(0.00001,0.02340)(P=0.04)and normal tissues,0.00092(0.00001,0.00455).(P=0.0002);however,there was no significant difference between the expression level in colon cancer tissues and the expression of adjacent normal tissues(P=0.108>0.05);3.The relative expression levels of long-chain non-coding CCAT1 and H19 in colon cancer were not statistically different in different groups,such as age(median age was 65 years old,65 years old group,<65 years old group),gender,lesion invasion,serous membrane and lymph node metastasis or not.;4.The expression level of LncRNA CCAT1 in colon cancer cell lines HT29 and HCT116 was significantly higher than that in normal colon cell lines NCM460(P<0.05),and silencing the expression level of CCAT1 in colon cancer cell lines HT29 and HCT116 by siRNA technology could significantly inhibit the proliferation of colon cancer cells.Conclusion 1.Long non-coding RNA CCAT1 and H19 may be involved in the occurrence of precancerous lesions;CCAT1 also plays a key role in the development of colon cancer.2.The expression levels of long non-coding RNA CCAT1 and H19 were not significantly correlated with the age,gender,tumor invasion depth and lymph node metastasis of colorectal cancer patients.3.The high expression of long non-coding RNA CCAT1 in colon cancer cells promotes the proliferation of colon cancer cells and is an effective target for the treatment of colon cancer.