The Study Of Extracting And Preparing Process, Quality Control And Hypoglycemic Action In Polysaccharide Of Gynura Divaricata(L.)DC

Objective:This paper is aimed to prefer the conditions of extraction and purification from Gynura divaricata(L.)DC and establish the quality standards of the polysaccharide.Investigating the different effective extracts(aqueous and alcohol extract ? crude polysaccharide19.5%) which influent the blood sugar?serum in sulin and lipid metabo Lism in diabete rats.Methods: We took the method of single factor,Box-Behnken response surface and Plackett-Burman experiment to screen the extraction and purification process of the polysaccharide from Gynura divaricata(L.)DC, then verified the prcess by three batches of samples of Gynura divaricata(L.)DC.We determined the physical and chemical properties Gynura divaricata( L.) DC polysaccharide by colorimetry,chemical analysis and HPLC method, furthemore,we used phenol-sulfuric acid method and a more measured assessment method to study the polysaccharide content.We repeated the model of type 2 diabetes of rats through high-sugar and high-fat diet combined with repeated Low dose intravenous injection of alloxan,then feeding intragastric administration of differernt extracts(aqueous and a Lcoho L extract ? crude po Lysaccharide19.5%?refined po Lysaccharide91.5%),then measured the blood sugar?serum in sulin and Lipid metabolism and so on by the method of kit ? chemical and pathologica L slide methods.Resu Lts: The different extracts had differernt effects on type 2 diabetic rats,the aqueous extract?crude polysaccharide can significantly reduce blood glucose in type 2 diabetes model,increase the liver and muscle glycogen content,T-SOD increased, MDA reduced significantly.We used 10 times the amount of purified water dissolved the polysaccharide, through the XDA-7 macroporous resin,the injection volume was 1.0g/g(crude drug/resin vo Lume),the diameter and height ratio of 1:6,eluent amount 5BV, a f Low rate of 1m L/min.The purified solution was added ZTC Natura L Clarifying Agent, the sequence is that the component B first Ly, the temperature is 80?,the volume fraction of 6%, 2h,then added the ZTC A component secondly, the temperature is 60?, the volume fraction of 3%,1h,centrifuged supernatant sample decolorization 1 macroporous resin, a flow rate of1 m L/min. Purification dialyzed, the polysaccharide yield was 0.14%,the content was91.5%.The results of verifying showed that three experiments polysaccharide yield is 0.01%,the RSD% polysaccharide content is 2.05%.The quality control results is that the color of the polysaccharide is white,amorphous powder, solub Le in water, no reduciton,no free monosaccharide,the monosaccharide composition conclued mainly D-anhydrous glucose, arabinose, galactose. The determination of polysaccharide content of galacturonic acid,glucuronic acid,mannose,phenol-sulfuric acid method was 91.5%.Conc Lusion: The crude polysaccharide of Gynura divaricata( L.) DC can significantly lower the blood sugar levels in rats of diats and improve glucose tolerance,protecting is Let cells. The extraction and purification process is reasonable and practiable,the yield and the content of polysaccharide RSD% is 2%;The QC method of polysaccharide was stable and controllab Le,which can accurately control the quality of polysaccharide, which laied the foundation for further development and utilization of Gynura divaricata(L.)DC.