The Effect Of The Overexpression Of SERPINA3 For MGC803 Cells

Gastric cancer is one of the most frequently occurring malignancies in China. Although great improvement has been made in diagnosis and treatment of gastric cancer, the five-year survival rate of the overall patients is still very low and less than 15%. It is known that carcinogenesis of gastric adenocarcinoma is a multi-stage process and the result of multistep accumulation of epigenetic alterations and genetic. 243 differentially expressed proteins were identified from normal gastric mucosal gland, atypical hyperplasia, gastric poorly differentiated adenocarcinoma, lymphaden metastasis gastric adenocarcinoma by laser capture microdissection(LCM) combined with Quantitative proteomics. The expression of SERPINA3 protein is progressively downregulation.To investigate the effect of the overexpression of SERPINA3 for MGC803 cells, we took gastric poorly differentiated mucinous adenocarcinoma cell line MGC803 as the study object, established MGC803 cell line of stable overexpression of SERPINA3(MGC803/pc DNA3.1-SERPINA3), and then measured the ability of cellular proliferation, apoptosis and cell cycle of MGC803 cell of SERPINA3 overexpression by drawing cellular growth curve, colony formation, soft agar formation, and flow cytometry(FCM). The results showed that: 1. The cell growth was significantly lower, and the number of plate colonies and soft agar colonies was significantly decreased MGC803/pc DNA3.1-SERPINA3 cells. 2. The rate of apoptosis significantly was increased, and the cellular proliferation index was decreased in MGC803/pc DNA3.1-SERPINA3 cells. The results demonstrated that the overexpression of SERPINA3 induced cellular apoptosis and decreased the cellular proliferation index to inhibited cellular proliferation.