LncRNA RP11-19119.4 Promotes Migration And Invasion Of Prostate Cancer Cell PC-3 | Posted on:2017-07-24 | Degree:Master | Type:Thesis | Country:China | Candidate:J Wang | Full Text:PDF | GTID:2334330491959887 | Subject:Urology | Abstract/Summary: | PDF Full Text Request | Objective:To explore the effect of lncRNA RP11-191L9.4 on the proliferation, migration and invasion ability of prostate cancer PC-3 cells.Methods:qPCR assay was used to confirm the low expression level of RP11-191L9.4 after the transfection of RP11-191L9.4 siRNA.MTT assay and colony-forming unit assay were performed to evaluate the effect of RP11-191L9.4 knock-down on PC-3 cell proliferation. Transwell migration assay was used to evaluate the effect of RP11-191L9.4 knock-down on the migration ability of PC-3 cells. Matrigel invasion assay was used to evaluate the effect of RP11-191L9.4 knock-down on the invasion ability of PC-3 cells.Results:The expression level of RP11-191L9.4 was significantly knocked down by the transfection of siRNA.MTT assay and colony forming unit assay showed that reducing the expression of RP11-191L9.4 can significantly weaken the proliferation ability of PC-3 cells. The result of transwell migration assay indicated that reducing the expression of RP11-191L9.4 in PC-3 cells obviously suppressed its migration ability. The result of Matrigel invasion assay demonstrated that reducing the expression of RP11-191L9.4 significantly suppressed the invasion ability of PC-3 cells.Conclusion:RP11-191L9.4 level in PC-3 cells can be efficiently knocked down with the transfection of RP11-191L9.4 siRNA, and knock down the expression level of RP11-191L9.4 can significantly suppress the the ability of proliferation,migration and invasion in PC-3 cells. | Keywords/Search Tags: | LncRNA, RP11-191L9.4, prostate cancer, cell proliferation, cell migration, cell invasion | PDF Full Text Request | Related items |
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