Classification Of Traditional Chinese Medicine Materials Based On The Unification Of Drug Materials

Objective:This topic through the study of the nature and changes in the nature of things,Reveal the drug release mechanism of traditional Chinese medicine pills,In order to control the quality of pills,ensure the clinical efficacy,the interpretation of the theory of traditional Chinese medicine to provide a scientific basis for the use of Chinese traditional medicine;Through the classification of materials of Chinese traditional medicine,complete the study of《The course of traditional Chinese medicine materia》,To provide the basis for the interpretation of traditional Chinese medicine dosage forms and the development of manufacturing industry;Complete the drug release mechanism of traditional Chinese medicine pill,to provide a research model of TCM system.Method: Supplementary material isthe basis of solid preparation drug release.Cluster analysis and correlation analysis combine with the unify of Drugs and accessories are used in this study.refer to the《Chinese pharmacopoeia》of 2010 Edition,The traditional Chinese medicine materials using the pill according to the frequency from high to low ranking,The specific research is as follows:1.representative drug screening and determination refer to the《Chinese pharmacopoeia》of 2010 Edition,The traditional Chinese medicine materials using the pill according to the frequency from high to low ranking,A total of 316,Therefore,in general,it is determined that part of Chinese herbal pieces as the research object.2.Determination of the content of main components of Chinese traditional medicine materials.The content of crude fiber was determined by acid washing method.Put the sample into the constant temperature drying box under the temperature of 95℃ Celsius drying 5h,To constant weight into the cooler to cool,precisely called 2.00 g dry sample,into the 250 ml triangle,adding 200 ml acidic detergent,connection backflow device,put the flask on regulating electric furnace,the contents in 5 ~ 10 min in boiling, the immediate time,allow the contents to keep the state of the micro boiling1.5h around,remove the triangular bottle,the content material is dumped into a known weight of glass crucible filter,the natural filtration.First with a glass rod agitating the filter residue,then 90-100℃ hot water washed three times,the total amount is 250 ml,which part of hot water washing flask,using acetone residual washing several times,until the filtrate colorless,circulating pump pumping dry residue in acetone,filter together with the residue into 100 ℃,constant temperature drying box drying 2h around,cooling after weighing.Determination of starch content in this kind of materials by dual wavelength method.The starch material was placed in the air blast drying box,and the moisture content of 5h was measured in the test sample at 105 ℃ centigrade W1.The dried sample into a Soxhlet fat extractor,adding ethyl ether,refluxing 2h degreasing treatment,Added concentration of 80 percent ethanol,Heat reflux 2h for removal of sugar and fat,And then put in the air blast drying oven drying,cooling,weighing,measuring the fat and sugar content of 105 ℃ W2.Accurate dehydration and dehydration of sugar samples 0.1000 g,Add a few drops of ethanol wetting in 50 ml beaker,Join the 0.1mol/l concentration of KOH 10 ml,10 min dissolved in boiling water,add distilled water volume to 50 ml,Mixing the liquid above,Draw 4ml of sample solution,adding 20 ~ 30 ml double distilled water,0.1 mol/l HCL with a concentration of PH to 3.5,plus 0.2ml iodine reagent,fixed capacity to 50.00 ml,Mixing the liquid above,Quietly placed 20 min,With double distilled water added 0.1 mol/l HCL and iodine reagent blank,color glass by 1cm,spectrophotometric determination of absorbance by UV,Then according to the regression equation were calculated sample solution of amylose starch content and amylopectin concentration calculation experimental materials of amylose,amylopectin and total starch content.3.Determination of the parameters of traditional Chinese medicine materials Moisture absorption(m)% : The bottom(T=25℃,RH=75%)of the sodium chloride is filled with a saturated solution of the dryer into a constant temperature incubator 25.In the weighing bottle with constant weight,the material is put into the material about 3mm thick,and the accurate weighing,the opening and the cover are arranged in the dryer,1,2,4,6,12,24,36,48,respectively.144 h weighing,Calculation of moisture absorption rate of(m)%=(weight after moisture absorptionweight before the weight)/ moisture absorption weight x 100%.Water absorption(w)%: Accurately weighed 0.200 g materials are arranged in a beaker,add 100 ml water,room temperature soaking for 1h,400 mesh nylon filter and static 5min after weighing.The calculation method of water absorption is 0.2 times the weight of the water after less than 1 of the final multiplied by 100%.Water swelling ratio(d)%: Take a suitable amount of material slowly even join in the test tube,each kind of material was added to a graduated tube 2ml,during which no oscillation tube,to prevent bottle material filling the tightness of the inconsistent,add appropriate amount of water,soaking for 1 hour,so that the material fully expanded to a certain scale,The calculation method of the water absorption expansion rate is the 100 of the difference of the original scale after the expansion of the scale and the difference of the original scale.Specific viscosity(e)%: Take the material and add water to make every1 m L containing 0.2g suspension,According to 《China Pharmacopoeia 》2010 two Annex VI G second method of the determination of viscosity(rotary viscometer)were measured.4.classification using the measured material parameters(cluster analysis)Clustering analysis is an analysis method according to the characteristics of the research object according to the standards of research objects are classified.It makes data objects within the group with the highest similarity,and group has great differences.The cluster analysis can be in without a priori classification through the observation to classify the data.This topic fully uses the SPSS20.0 clustering analysis method,uses the measured Chinese medicine material parameter to originally belongs to the fiber,the polysaccharide,the starch three big categories to carry on the cluster analysis.Results : Through the experimental study,the following results were obtained:1.Using hierarchical clustering analysis method and fast clustering result is the polysaccharide content is relatively high for class 1,polysaccharide content in the middle is divided into 2 categories,fiber content relatively high for 3,and relatively low fiber content and polysaccharide content and starch points into four categories.2.The use of spss20.0 correlation analysis and moisture absorption rate of crude polysaccharide content and viscosity become significantly positive correlation and the coefficients of the two-sided test respectively 0.01 and0.001;crude fiber content and the water absorption and expansion rate significantly positive correlation and coefficient of two-sided test respectively 0.003 and 0.017;expansion of total starch content and water absorption rate significantly positive correlation,coefficient of two-sided test was 0.029;amylopectin content and ratio of viscosity is significantly positive correlation,coefficient of two-sided test is 0.015.Conclusion: Through the experiment and analysis,the following conclusions are obtained:Classification results using material parameters,and the use of principal component content classification results are broadly consistent.Show different medicinal materials;principal component,the physical properties are not the same,and therefore will on traditional Chinese medicine pill dissolution effect,so the use of material parameters of medicinal materials classification is feasible.