| Objectives: To observe the curative effect of Arsenic trioxide perfusion liver through hepatic artery and portal vein combination biomembrane isolation around liver in VX2 rabbit.Methods:32 New Zealand white rabbits were opened to inoculate VX2 models. 30 VX2 rabbits successfully inoculated were divided randomly into 4groups. Group A(6): carried out an intravenous injection on ear margin, without opening and catheter. Group B(8): TAE combined with Arsenic trioxide perfusion. Group C(8): TAVE combined with Arsenic trioxide perfusion. Group D(8): TAVE, Arsenic trioxide perfusion, combined with antiblocking isolation membrane around the liver. Two weeks after inoculating, operated the 30 rabbits to lay catheters. The alanine transaminase(ALT), aspartate aminotransferase(AST), tumor growth, lung metastasis, and survival time were analysed. Results of observational index expressed by mean number±standard deviation. Comparison among groups were as follows: adopted single factor variance for time points, LSD for any two among groups, variance analysis of repeated measurement data for changes among groups, Pearson Chi-square for pulmonary metastasis, Kaplan-Meier for survival analysis and log-rank for survival analysis among groups.Results: First, did CT scan 1 day before operation and operated the rabbits two weeks after establishment, and tumors of four groups were all successfully inoculated. The face of tumors were gray. Second, the ALT and AST were no statistically significant in each group at 1 day before operation(P>0.05). At the 3th day after operation, the ALT, AST of group B, C, D was significantly higher than that of group A(P<0.05), and the ALT, AST of group C,D is the highest. At the 7th day after operation, the ALT, AST of group B, C, D began to fall. But its' were still higher than that of group A(P<0.05),and the ALT, AST of group C were higher than that of group B(P<0.05), the ALT of group D higher than that of group B(P<0.05). At the 14th day after operation, the ALT?AST of group A increased significantly, but the ALT, AST of group B, C, D still to fall. Third, the tumor volume were no statistically significant in each group at 1 day before operation(P>0.05). At the 14 th day after the operation, the average tumor volume of group B, C, D were still smaller than that of group A(P < 0.05). And the tumor of group D were the smallest(P < 0.05). Four, no lung metastasis were found in each group at 1 day before operation. At the 14 th day after operation,the lung metastasis of group A was the highest(P < 0.05),and the lung metastasis of group D were less than that of group B, C(P < 0.05). Finally, the median survival time of each group were as follows: group A(21.17±1.60d), group B(24.80±1.47d), group C(27.00±1.41d), group D(32.29±2.56d). The survival time of group B,C,D was significantly longer than that of group A(P < 0.05). And the survival time of group D was the longest.Conclusions: Arsenic trioxide by intravenous injection can reduce the liver damage in a short time, but as time going by, it will affect the liver function seriously. This chemotherapy can not inhibit tumor growth, can not reduce the rate of lung metastasis effectively, and can not extend the overall survival of VX2 liver carcinoma rabbit. Comparing with Arsenic trioxide combined with TAE, Arsenic trioxide combined with TAVE can aggravate the liver damage in short time, but can not hasten the death of rabbit. These two methods in inhibiting tumor growth and reducing the rate of lung metastasis were about the same, but both better than systemic chemotherapy. Arsenic trioxide combined with TAVE can extend the survival time. TAVE, Arsenic trioxide perfusion, combined with antiblocking isolation membrane around the liver can better extend the survival time, and it is a more effective method to treat liver cancer. |
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