The Role Of RNA Helicase DHX32 In Angiogenesis Of Colorectal Cancer

Objective:We observe the role of RNA helicase DHX32 in colorectal cancer angiogenesis,and to explore the regulatory mechanism.Our objective is to understand the pathogenesis of colorectal cancer,and hope to provide a novel therapy-target and theoretical foundation for colorectal cancer.Methods:1. The Vefg-a m RNA of colorectal cancer cells were detected by real-time PCR;VEGF protein in cell culture supernatant were detected by ELISA; 2. Ch IP technology were used to analyze DHX32 and ?- catenin regulating on Vefg-a gene transcription; 3. MTS,wound healing assay,Matrigel Invasion Chamberl and in vitro angiogenesis assay were performed in vitro to test HUVEC cell proliferation,migration,invasion and angiogenesis,respectively;4. DHX32,CD31 and ?-catenin protein were detected by immunohistochemistry in colorectal cancer tissues; 5. T test were used with two groups of independent sample,and P value were calculated; Spearman rank correlation were to analyze the correlation between DHX32 expression with microvessel density(MVD) and ?–catenin, respectively; Log-rank test were to analyze the relationship between DHX32 expression spectrum in colorectal cancer tissue microarray with patient survival.Results: 1.Overexpressed DHX32 raised Vegf-a m RNA and protein level(P<0.001), depleting DHX32 downgraded the Vegf-a m RNA and protein level(P<0.001); 2.DHX32 may through the ?-catenin regulated Vefg-a gene transcription;3.Overexpressed DHX32 promoted HUVEC cell proliferation,migration,invasion and angiogenesis; depleting DHX32 suppressed HUVEC cell proliferation,migration, invasion and angiogenesis; 4.DHX32,?-catenin and CD31 were overexpressed in colorectal cancer tissues;DHX32 expressed with MVD(R=0.742,P<0.001) and ?-catenin(R=0.549,P<0.01)were positive correlation; 5. The patients with DHX32 overexpressed in colorectal cancer tissue were significantly lower than patients with low expression in 5 year survival rate(P<0.05).Conclusion: 1.DHX32 may through the ?-catenin regulated Vefg-a gene transcription, thus promotes VEGF expression. 2.DHX32 promote colorectal cancer cell angiogenesis. 3.DHX32 overexpress in colorectal cancer tissue,and is positive correlation with MVD; The patients with DHX32 overexpressed are low obviously in 5 year survival rate.DHX32 abnormal expression may can be used as a prognostic indicator.