| Objective:Detect the level of DHX32 protein in colorectal cancer cell lines;investigate the effect of DHX32 on angiogenesis of tumors in nude mice model which were injected SW480 cells;calculate the expression of DHX32 in colorectal cancer tissues,explore the correlations between DHX32 and ?-catenin?angiogenesis,study the associations between the expression of DHX32 and pathologic features and the prognostic value of DHX32 on patients with colorectal cancer,for providing a novel thread for assisting in diagnosing?therapeutics and prognostic evaluation of colorectal cancer.Methods:1.Prepared cell slides,the expression and location of DHX32 in four kinds of cells: three kinds of colorectal cancer cells(SW480?SW620 and DLD-1)and a normal colorectal cell(CCD-18Co)was detected by immunochemistry and immunofluorescence.2.Prepared the tumor tissues of nude mice to investigate the value of DHX32 to angiogenesis by immunohistochemistry(vascular vessels were stained by anti-CD31,CD31 is a marker for endothelial cells and the number of CD31 represents the ability of angiogenesis).3.Collected a total of 139 colorectal cancer tissues from clinical,calculated the expression of DHX32 by immunohistochemistry,meanwhile,the level of ?-catenin and CD31 were carried out.4.Two groups of non-rank test were used,and P value were calculated,because the data was not in homogeneity of variance;Analysis of the correlation between the expression of DHX32 and ?–catenin,CD31 were conducted using the spearman rank correlation;The association between the level of DHX32 and pathologic features of colorectal cancer were examined by chi-square test and multivariate logistic regression analysis;The relationship between the expression of DHX32 and prognosis of colorectal cancer was estimated by log-rank test.Results:1.DHX32 were upregulated in colorectal cancer cells were all higher.2.Ablation of DHX32 reduced the microvessel density of tumors of nude mice.3.Compared to the adjacent normal tissues,the expression of DHX32,?-catenin and CD31 were up regulated in human colorectal cancer tissues.4.The level of DHX32 in colorectal cancer tissues was positive correlated with ?-catenin(r=0.515,P<0.001)and CD31(r=0.602,P<0.001).5.The level of DHX32 was associated with the differentiation of colorectal cancer,the tissues with poor/moderate histologic grade were found to have higher expression of DHX32.6.The overall survival time and metastasis free survival time of colorectal cancer patients were shorter in the groups exhibiting high DHX32 expression,compared with the group with low DHX32 expression.Conclusions:1.The level of DHX32 protein was up regulated in the colorectal cancer cell lines,and mainly located in cytoplasm.2.Ablation of DHX32 inhibits the angiogenesis of tumor in the nude mice.3.DHX32 is upregulated in colorectal cancer tissue,and is positive correlated with the expression of ?-catenin?CD31,and the tissues with poor/moderate histologic grade were found to have higher expression of DHX32.Additionally,the overall survival time and metastasis free survival time of colorectal cancer patients were shorter in the groups exhibiting high DHX32 expression,compared with the group with low DHX32 expression.The abnormal expression of DHX32 in colorectal cancer tissues may be closely related with the progression of colorectal cancer,and is expected to be a biomarker to help in diagnosing?clinical therapeutics and prognostic evaluation of colorectal cancer. |
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