Fabrication Of Osteogenic Core-shell Nanofibers Of Silk /Poly(L-lactic Acid-co-?-caprolactone) Loaded With ICA By Coaxial Electrospinning And Its Characterization

Nanofiber material has a lot of potential values in fieNanofiber material has a lot of potential values in field of tissue engineering,drug sustained-releasing and drug-delivering due to its small diameter, high surface area and great biocompatibility.Not only can it reduce the distant of drug diffusion,but also promote drugs ld of tissue engineering,drug sustained-releasing and drug-delivering due to its small diameter, high surface area and great biocompatibility.Not only can it reduce the distant of drug diffusion,but also promote drugs dissolution and the rate of carrying drugs,such a benefit like provide a good environment for cell adhesion and growth.In recent years, three-dimensional nano fiber which made by coaxial electrospinning has been widely concerned. Coaxial electrospinning developed from traditional electrospinning technology. This technique has been widely used in the preparation of three-dimensional nano fiber scaffold with Core / shell structure which can be handled easily.The shell structure is a photosensitive polymer material with atisfactory biocompatibility. The core structure is a material with outstanding mechanical property.When the core layer was equipped with the following substances(light, electricity, chemical active substances), the fibers can be obtained many functions like drug releasing, catalysing and sensing. Nanofibers with core / shell structure have shown great potential in tissue engineering, drug sustained-releasing, drug-delivering and other biomedical fields.The rapid development of tissue engineering has brought new hope for the treatment of periodontal disease. The periodontal tissue engineering include: scaffold materials, seed cells and growth factors.Summary the research results and the direction of it. Scholars have made a lot of exploration and research in the applications of natural polymers and high molecular polymer. The purpose of such efforts was to find an ideal scaffold material for periodontal tissue engineering and meet the clinical needs.Our research group have done some tentative exploration and some achievements have been obtained.The goal of this experiment is to utilize coaxial electrospinning technique to make a composite nano-biological material as a new scaffold material that icariin(ICA) as the core and Silk fibroin(SF), poly(lactic-co- e-caprolactone)(PLCL) as shell for periodontal tissue engineering. Experiment is to determine the best spinning parameters, make nanofibers embranes with diffrent flow raio of core-to-sheath(1:6; 1:8; 1:10; 1:12).Three-dimensional nanofiber scaffold materials has been prepared by cross linking reaction with glutaraldehyde vapor.Explore its physicochemical characterization by scanning electron microscopy(SEM), transmission electron microscopy(TEM), contact angle measurement, X-ray diffraction(XRD), thermogravimetry(TG) measurement and mechanical property test to evaluate properties of coaxially electrospun fiber membranes obtained at different core–shell velocity ratios. the ICA–SF/PLCL fibers possessed higher quality, morphology was favorable, smooth, and overlapping with appropriate pore size and porosity,each group exhibited an obvious core–shell structure at 15 kV, 15 cm working distance and the concentrations of the shell solution SF/PLCL(30:70, wt) and core solution ICA were 8 % and 10-6 mol/L, respectively,as verified by scanning electron microscopic observations of their surface.The diameter of the drug-loaded nanofibers increased as the velocity ratio of the core–shell decreased. No obvious differences in the contact angle of the nanofibers were found. The decreased core-to-shell velocity ratio enhanced the mechanical properties of the coaxially electrospun nanofibers.In vitro release experiments results indicated that coaxially electrospun fiber membranes exhibited better stable sustained-release curve,which were obtained at 1.0 mL/h shell solution flow rate,0.1 mL/h core solution flow rate.Overall, This study aimed to prepare silk /poly(L-lactic acid-co- ?-caprolactone) by blending composite nanofiber scaffolds with Icariin using the coaxial electrospinning technique,with silk /poly(L-lactic acid-co-?-caprolactone) as sheath liquid and an electrospinnable solution of ICA as core fluid.Experiments show various excellent physical and chemical properties of it.It can be used as a kind of reservoir type drug release material for periodontal regeneration.