Colchicine Modified Selenium Nanoparticle Targeted MCF-7 Cell And Triggered Its Apoptosis

Selenium nanomaterials possess plenty of biological activities, Which can be obtained by surface modification of active components. Detection of cytotoxicity of drugs using AFM is a new emerging drug efficient evaluation method. In this paper, we prepared colchicine modified selenium nanoparticles(COL-Se NPs). The successful formation of COL-Se NPs were examined by UV-VIS spectrometer Infrared spectrometer, HR-TEM and Nano-ZS Nano particle sizer. And MTT, flow cytometry, AFM, CLSM and other methods were used to investigate the toxic effects and mechanism of COL-Se NPs against MCF-7 cells and anti-angiogenesis, We obtained some insightful results as described below:1. We prepared spherical COL-Se NPs with an average diameter of 70 nm. In this system, CS was used as the stabilizing agent and surface modification. Colchicine molecules were successfully coupled onto selenium nanoparticles through the reaction between the carboxyl from the colchicine molecules and the surface of selenium nanoparticles.2. The result showed that COL-Se NPs could efficiently inhibit the growth of MCF-7 cells in a dose-dependent manner but had low cytotoxic effects on normal cells(MCF-10A). Flow cytometry detected that COL-Se NPs treatment resulted in a dose-dependent accumulation of MCF-7 cells in S phase. CLSM imaging demonstrated that COL-Se NPs significantly disrupt the microtubule network and inhibit the formation of spindle. The changes of morphology and ultra-structure properties detected by AFM indicated that COL-Se NPs could induce the change of MCF-7 cell morphology?change the membrane roughness and particles size.3. CCK-8 assay showed that COL-Se NPs could remarkably inhibit the growth and proliferation of HUVEC cells. Annexin V/PI-based on flow cytometry analysis showed that of COL-Se NPs could induce HUVEC cell death through apoptosis and necrosis induction. In addition, COL-Se NPs could block the migration of HUVEC cells in scratching assay. At the same time, COL-Se NPs could block tube formation, and it also influenced the formation of blood vessels in the chicken chorioallantoic membrane(CAM).Above all, COL-Se NPs as a potential anti-cancer drug which can destroy microtubule dynamic balance, inhibit cell proliferation and induce apoptosis of MCF-7 cells.. Additionally, COL-Se NPs can inhibit the proliferation of HUVEC cells, so as to achieve the anti-angiogenesis. These results show that COL-Se NPs can be as a kind of potential anti-cancer drug.