Quantitative Analysis Of Ops And Pigment Epithelium-derived Factor In The Serum And Aqueous Humor With Diabetic Retinopathy

Objective To investigate the relationship between epithelium derived factor pigment(PEDF) in serum and aqueous humor and oscillatory potentials(OPs) in various diabetic retinopathy and to provide an objective basis for the early diagnosis of diabetic retinopathy(retinopathy diabetic, DR)?Methods Aqueous humor samples were collected from 40 eyes of 40 type 2 diabetes patients,including 19 eyes without diabetic retinopathy and 21 eyes with diabetic(11 eyes with NPDR and 10 eyes with PDR), Twenty aqueous samples from 20 cataract patients without other ocular or systemic diseases served as controls. The PEDF levels in serum and aqueous humor were measured by enzyme-linked immunosorbent assay(ELISA),The total amplitude of Ops in the whole retina was detected by the Roland visual electrophysiological test instrument of the German CONSULT ROLAND company?Results 1.the concentration of PEDF in the NPDR and PDR group was significantly lower than that in the patients with type 2 diabetes mellitus(P=0.04,P=0.02);2.there was no significant difference between NPDR group and PDR group(P=0.34);3.The PEDF levels in serum in eyes without diabetic retinopathy [(12.13±1.02) ng/ml, P<0.01] was significantly higher than that in controls [(8.89±2.88) ng/ml]; The PEDF Ievel in eyes with NPDR [(13.76±1.05)ng/ml, P<0.01] and [PDR(16.40 ±0.91) ng/ml, P<0.01] were significantly higher than that in controls [(8.89±2.88) ng/ml]; the concentration of PEDF in the patients with type 2 diabetes mellitus was significantly lower than that in the NPDR and PDR group(P<0.05);4.The Ops level in eyes without diabetic retinopathy[(164.56±37.41)?V,P<0.01]was significantly lower than that in controls[(237.96 ± 35.96) ?V]; The Ops level in eyes with NPDR[(79.80±19.89)?V, P<0.01]and PDR [(28.55±17.47)?V, P<0.01]were significantly lower than that in controls[(237.96 ± 35.96) ?V]; There were significant differences in the different Ops group(P<0.01);5.The PEDF concentration in the aqueous humor of the diabetic retinopathy was a typical positive correlation with the total amplitude of Ops(r=0.905, P<0.01);6.Changes of PEDF concentrations in the aqueous humor of the diabetic retinopathy and the concentrations of PEDF in serum were positively correlated with the concentration of serum(r=-0.712,P<0.01);7.The PEDF concentration in the serum of the diabetic retinopathy was a typical positive correlation with the total amplitude of Ops(r=-0.686,P<0.01).Conclusion The concentration of PEDF in aqueous humor and the total amplitude of Ops were positively correlation with the diabetic retinopathy, which could provide an objective basis for the early diagnosis of diabetic retinopathy and the prediction of the progression of the disease. The concentrations of PEDF in aqueous humor and serum concentrations of PEDF were negatively correlated with the concentrations of PEDF in each phase of diabetic retinopathy, and the changes of peripheral blood concentration may be the basis for prediction of early diagnosis and progression of diabetic retinopathy.