Anti-Cancer Bioactive Peptide ACBP Potential Chemotherapy On The Experimental Mice Attenuated Sensitization Effect And Mechanism Research

Objective To discuss the short-term intermittent application of anticancer peptides combined with oxaliplatin in human colorectal carcinoma xenograft tumor therapeutic effect and its mechanism. Methods Nude mice were implanted with human colorectal carcinoma HCT-116 cells. All tumor-bearing mice were randomly divided into five groups(n=6) and treated with vehicle, 0.5ml /kg 0.5%glucose, 0.5ml anti-cancer bioactive peptides solution, 8 mg/kg oxaliplatin, 12 mg/kg oxaliplatin and their combination(0.5ml /kg anti-cancer bioactive peptides + 8 mg/kg oxaliplatin) by ip injection for 2 times every week individually. After continuous administration of drug treatment for 4 times, the weights of nude mice were recorded, the stripping tumor weight was monitored, and the tumor volume and tumor inhibitory rates were calculated.The enucleation of eyeball for taking blood and the tumor cell cycle and apoptosis rate were assayed by flow cytometry. The expression of transplantation tumor tissue apoptosis-associated proteins of cyt-c, Caspase-3/8/9 was analyzed by Immunohistochemistry and the gene expression of it was detected by RT-q PCR. Results(1).Although the ACBP-S and ACBP-S+L-OLP group's weight increased and active but L-OLP group's weight decreased and active two weeks after the drug treatment compared to the control, there was no statistical differences(P>0.05). Compared to the L-OLP's weight, there was statistical differences between ACBP-S, ACBP-S+L-OLP,H-OLP and L-OLP(P<0.05) and that ACBP-S and ACBP-S+L-OLP group showed a good diet and activity.(2).Compared to the GG's group transplantation tumor volume, ACBP-S+L-OLP, H-OLP and ACBP-S's transplantation tumor volume showed smaller size, lighter weight, but there was no statistical differences between ACBP-S+L-OLP, H-OLP and ACBP-S(P>0.05). There were no statistical differences of transplantation tumor volume and weight between L-LOHP, ACBP-S and GG(P>0.05), but statistical differences between ACBP-S+L-OLP and GG(P<0.05).(3).The flow cytometry results showed that the transplanted tumor apoptosis rate of ACBP-S+L-OLP, L-OLP, ACBP-S increased compared to the GG, however there was no statistical differences between ACBP-S+L-OLP, L-OLP and ACBP-S(P>0.05). There were no statistical differences of transplanted tumor apoptosis rate between L-OLP, ACBP-S and GG(P>0.05), but statistical differences between ACBP-S+L-OLP and GG(P<0.05).(4).The immunohistochemical results showed that the protein expression of cyt-c, Caspase-3/8/9 of ACBP-S+L-OLP, L-OLP, ACBP-S increased compared to the GG, however there was no statistical differences between ACBP-S+L-OLP, L-OLP and ACBP-S(P>0.05). There were no statistical differences of protein expression of cyt-c, Caspase-3/8/9 between L-OLP, ACBP-S and GG(P>0.05), but statistical differences between ACBP-S+L-OLP and GG(P<0.05).(5).The RT-q PCR results showed that the gene expression of cyt-c, Caspase-3/8/9 of ACBP-S+L-OLP, L-OLP, ACBP-S increased compared to the GG, however there was no statistical differences between ACBP-S+L-OLP, L-OLP and ACBP-S(P>0.05). There were no statistical differences of gene expression of cyt-c, Caspase-3/8/9 between L-OLP, ACBP-S and GG(P>0.05), but statistical differences between ACBP-S+L-OLP and GG(P<0.05). Conclusion(1). Short-term intermittent alone ACBP-S has a certain role in inhibiting tumor growth, short-term intermittent use in combination with chemotherapy drugs ACBP L-OLPfor L-OLPsignificant sensitizing effect.(2). Short-term intermittent application ACBP-S has improved the quality of life of nude mice role. Short-term intermittent use ACBP-S combination with chemotherapy(L-OLP) can significantly reduce the side effects of chemotherapy, while significantly improving the quality of life in nude mice.