Effects Of Hepatic Electrical Stimulation On Gastric Motility And Its Possible Mechanism In Carbon Tetrachloride–related Liver Injury

OBJECTIVE: 1. To investigate the changing of gastric motility in carbon tetrachloriderelated liver injury model and it's possible mechanism.2. To explore the underlying effects of hepatic electrical stimulation on gastric motility in carbon tetrachloride-related liver injury mode and it's mechani sm.METHODS: 1. Forty-eight male, 6-8 week-aging SD rats were randomized into normal control, solvent-control and CCL4 three groups. According to the time of abdominal injection, every group was divided into three groups(twice, three times, four times). After the total injection, the HE dyeing was employed to see the Pathological changes of hepatocytes; the activity of the ALT and AST were measured by microplate method; gastric emptying was studied by phenol red method; the expression of HNF-1?and TGF?-1m R NA in liver, TGF?-1 and n NOS m NA in gastric antrum were assessed by RT-PCR, the protein of HNF-1?and TGF?-1 in liver, TGF?-1 and n NOS in gastric antrum were detected by Western Blot. 2. Fifty-six male, 6-8 week-aging SD rats were randomized into normal, oil, electrode control, true stimulation and sham stimulation groups. These five groups were divided into acute stimulation group(2-week stimulation) and chronic stimulation group(4-week stimulation) according to the duration of electrical stimulation. Then the HE dyeing was employed to see the pathological changes of hepatocytes; the activity of the ALT and AST were measured by microplate method; gastric emptying was studied by phenol red method; the expression of HNF-1?and TGF?-1m RNA in liver, TGF?-1 and n NOS m NA in gastric antrum were assessed by RT-PCR, the protein of HNF-1?and TGF?-1 in liver, TGF?-1 and n NOS in gastric antrum were detected by Western Blot.RESULTS: 1. After the fourth time of injection of CCL4, the gastric emptying were severely delayed, hepatocytes were detected with pathological damage, the activity of ALT and AST in serum were measured high than control groups. The expression of HNF-1?m RNA and protein were attenuated and TGF?-1m RNA and protein were increased in liver. In gastric antru m, the m RNA and protein expression of TGF?-1 were ascended. On the contrary, the expression of n NOS declined. 2. After the acute electrical stimulation, the gastric emptying were increased compared with the sham electrical stimulation group and the activity of ALT and AST was depressed as well. The expression of HNF-1?m RNA and protein was a little increased, but there is no statistical significance compared with the sham stimulation group. So did the expression of the n NOS. Higher expression of TGF?-1 m RAN and protein expression were seen in true stimulation group compared with sham stimulation group and there is statistical significance. A more evident effects were discovered with chronic stimulation. And the increased HNF-1?and n NOS m RNA and protein expres sion were more significant.CONCLUSION: 1. The gastric emptying is delayed in carbon tetrachloride-related liver injury group,the mechanism may be associated the down-regulation of HNF-1?and n NOS and upregulation of TGF?-1. 2. Hepatic electrical stimulation is safe and has nothing to do with the liver pathology and biochemistry. 3. The delayed gastric emptying in CCL4-related liver injury rats can be ameliorated by hepatic electrical stimulation through up-regulating HNF-1?and n NOS and down-regulating TGF?-1.