The Research Of American Periplaneta Polysaccharide Extraction Process Optimization And Antitumor Effect

Objective: The different polysaccharide extraction technology of Polysaccharide from Periplaneta americana extract test,screening and optimization of Periplaneta americana polysaccharide extraction process,to create efficient Periplaneta americana polysaccharide extraction approach to provide technical support;with glucose as the control sample by phenol-sulfuric acid spectrophotometric method was used to detect the samples in the content of polysaccharide,and comparison of different extraction conditions obtained many sugar extraction rate differences;through animal test determination of Periplaneta americana polysaccharide on the immune function of mice and on the S-180 the inhibitory rate of tumor and other indicators,so as to provide reference data for Periplaneta americana of functional food and medicine development.Methods: The salt extraction,dilute alkali extraction and papain hydrolysis method to extract polysaccharide from Periplaneta americana,comparison of different extraction conditions of polysaccharide extraction rate,determine the Periplaneta americana polysaccharide optimum extraction conditions.Content determination of Polysaccharide from Periplaneta americana by spectrophotometric method with phenol sulfuric acid.Improving the immune function of mice and inhibiting the growth of S-180 tumor by using the polysaccharides isolated from the experiment.Results: The highest extraction yield of Periplaneta americana polysaccharide papain hydrolysis method,measured the polysaccharide content was 21.25%.Periplaneta americana polysaccharide optimum extraction conditions for A2B3C1,enzyme dosage for 200 g-g-1,extraction temperature is 60 ?,the extraction time was 1 h,extraction of Periplaneta americana polysaccharide content is the highest.Animal test indicated that polysaccharide from Periplaneta americana can appropriately increase the weight of immune organs,promote the thymus index and spleen index increased slightly,clearance index in mice and corrected phagocytic index increased,thus indicating that Periplaneta americana polysaccharide can increase the non-specific immune function,can improve the biological immune regulatory role.Periplaneta americana polysaccharide on the to the growth of S-180 sarcoma mice have certain inhibition,and in 200 ~ 600 mg / kg / D within the scope of the inhibitory rate of tumor with dose increasing,the optimal dose for 600 mg / kg / D,the inhibition rate of 47.13%.Conclusion: Through the study on the extraction technology of Polysaccharide from Periplaneta americana,different extraction conditions of Polysaccharide from Periplaneta americana extraction rate differences,and papain hydrolysis method suitable to extract polysaccharide from Periplaneta americana,the extraction rate and polysaccharide content were higher than those of salt extraction and alkali extraction and papain hydrolysis under controlled conditions is a kind of easy extraction process for industrial production and extraction of Periplaneta americana polysaccharide can effectively improve the immune function of mice with experimental animals and has better anti-tumor effect,can be used for its medicinal value of promotion.