The Effect Of Laser Micropore Porcine Acellular Demal Matrix On Vascularization Of Wounds With Full-thickness Burn

Objective: To explore the effect of laser micropore joint repeated freezing and thawing porcine acellular demal matrix on vascularization of wounds in rats with full-thickness burn. We strive to provide an ideal dermal scaffold for clinical.Methods : Frist step: preparation of joint laser micropore and freezing and thawing porcine acellular dermal matrix; we Detected of HE staining. The second step: The preparation of laser micropore porcine acellular demal matrix and ordinary punch porcine acellular demal matrix preparetions experiment was carried out. The specific method is as follows. laser micropore porcine acellular demal matrix and porcine acellular demal matrix were respectively embedded in the dorsum of eighteen rats. After 3 days, 1 week, 2 weeks and 3 weeks, the skin flap was performed with HE staining and CD31 immunohistochemical staining. The third step: And then carries on the composite skin transplantation experiment.Laser micropore porcine acellular demal matrix(A group) and porcine acellular demal matrix(B group) were respectively transplanted on the seventy-two rats with full-thickness burn after wound debridement;there were no dermal in the control group(C group). Then skin was transplanted into the rats of the three groups one week later. The repairation of dermal implant wound was recorded after 1,2,and 3 weeks respectively. At the same time, CD31, ?-SMA positive signals detected by immunohistochemical staining and HE staining were used to observe the effect of repairation.Results: The well-prepared laser micropore porcine acellular demal matrix was characterized by its porcelain white color, softness and good elasticity. In our three weeks experiment subcutaneous implant test showed that rat dermal matrix with good?compatibility, no rejection. CD31 marked positive blood vessels there was no difference between the two groups after 3 days(P>0.05).Laser micropore porcine acellular demal matrix were significantly more than the porcine acellular demal matrix after one week, two weeks and three weeks(P<0.01). The composite skin grafting test results: histological examination revealed that laser micropore porcine acellular demal matrix was totally devoid of epidermis and cellular components Group A has higher survival rate than group B. From 1th to 3th week, the number of vessels with CD31 and ?-SMA expression were continous increase in the three groups. The number of vessels with CD31 and ?-SMA expression in group A and B were than in group C(P<0.01), and it was significantly more in group A than in group B and group C(P<0.01). Dermal matrix implants implanted on the skin after a week. After one week grew well, But group B graft epidermis in survival After three weeks group A the skin confluent and the dermal matrix has been combined with wound completely, but group B skin grew well and beginning confluent, dermal matrix combined with the substrate in close.Conclusion: 1. Our experiment produced laser micropore porcine acellular demal matrix was characterized by its porcelain white color, softness and good elasticity. 2. laser micropore porcine acellular demal matrix with good compatibility, no rejection which can rapid vascularization. 3. Our experiment produced can be used as an ideal demal substitute in the composite skin grafting test results due to its ability of initiating revascularization rapidly that may increase the survival rate of the grafted skin.