| Objective:This study is to analyse the relationship between serum B cell activating factor(BAFF) and periodontal clinical indexs through the epidemiological investigation and biochemical detection, to detect the expression of BAFF and its receptors in human gingival fibroblasts(HGFs) and compare them in gingival tissue from chronic periodontitis patients and healthy volunteers in order to demonstrate whether BAFF are involved in the process of periodontitis. To explore the role of BAFF in the relationship between obesity and periodontitis through the analysis of the relationship between serum BAFF levels and obesity, and the measurement of the expression of BAFF, adipose differentiation gene and inflammatory chemotactic factor in HGFs after high glucose stimulation. Method:1. The plaque index(PLI), probing depth(PD), attachment loss(AL), bleeding index(BI) of selected teeth according to the community periodontal index(CPI), and other auxiliary examination results of middle aged and elderly population were examined. Serum BAFF levels were detected and the relationship between the serum levels of BAFF and periodontal indexs was analyzed.2. HGFs were collected from healthy adults and cultured. The expression of BAFF and its receptors B cell activating factor receptor(BAFF-R), B cell maturation antigen(BCMA) and transmembrane activator and calcium-modulating cyclophilin ligand interactor(TACI) were detected using flow cytometry and reverse transcription polymerase chain reaction(RT-PCR). B cells were taken as positive control. BAFF and its receptors expression were detected in gingival tissue from chronic periodontitis patients and healthy person using real-time PCR.3. According to body mass index(BMI), the study population was divided into 3 groups: normal, overweight and obesity. The relationship among serum BAFF level, periodontal indexs and other results were analyzed by one-way ANOVA.4. The expressions of BAFF, peroxidase proliferator activated receptor gamma 2(PPARγ2), suppressor of cytokine signaling 3(SOCS3) and CC chemokine ligand2(CCL2) were detected in HGFs via real-time PCR at 1 d, 3 d, 5 d, 7 d and 9 d after high glucose stimulation. Results:1. With the increase of PLI, PD and AL, the serum BAFF had an increasing trend(P>0.05). When BI≥2, serum BAFF level increased significantly(P<0.05).2. Flow cytometry showed that healthy adults HGFs almost didn’t express BAFF receptors. RT-PCR showed that BAFF m RNA level in gingival tissue from periodontal healthy adults was weak. BAFF receptors mRNA levels were very weak.3. Compared with healthy volunteers, the expression levels of BAFF and TACI mRNA in gingival tissues from patients with chronic periodontitis were significantly increased(P<0.05). BAFF-R and BCMA mRNA expression didn’t change(P>0.05).4. The general condition among the three groups wasn’t different. Blood routine indexes were in the normal range. Fasting blood glucose, triglyceride, total cholesterol, low density lipoprotein cholesterol and PLI, PD, AL among the three groups were no difference(P>0.05). BI in the obesity increased significantly(P<0.01). Serum BAFF levels in the obesity was higher than in the normal(P<0.05).5. High glucose stimulation induced BAFF mRNA expression increase in HGFs(P<0.05). PPARγ2 has an increased trend. SOCS3 increased significantly at 9 d(P<0.05). CCL2 significantly increase at 5 d, 7 d, 9 d(P<0.05). Conclusion:1. The correlation between BAFF and gingival bleeding index, high expression of BAFF and TACI in inflammatory gingiva indicate that BAFF is involved in the progress of periodontal inflammation.2. There is a correlation between BAFF and obesity, suggesting that BAFF plays an important role in the process of periodontitis in obese patients.3. High glucose can promote the trend of HGFs adipogenic differentiation, and enhance the expression of BAFF, SOCS3 and CCL2, which shows that BAFF may be involved in the regulation of the relationship between periodontitis and obesity. |
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