Analysis Of Correlation Between Methylene-Tetrahydrofolate Reductase Gene C677T,A1298C Polymorphism And Level Of Homocy-Steine Or The Severity Of Coronary Artery Disease

Objective: To investigate the relevance of the blood homocysteine levels and other cardiovascular disease independent risk factors.Analysis the correlation of MTHFR C677 T A1298C Polymorphism and HCY levels, HHCY, the incidence of coronary heart disease and the severity of coronary artery disease.To find out the susceptible genotype, allele and haplotype.Methods: We studied 947 inpatients in the Department of Cardiology in the Tianjin Chest Hospital.We collected of the blood pressure, cholesterol, smoking, diabetes and coronary heart disease risk factors and other biochemical assay indexs of clinical data and collected blood samples.Polymerase chain reaction restriction fragment length polymorphism(PCR-RFLP) were used to detect MTHFR gene C677 T, A1298 C gene polymorphism. And the plasma HCY level was detected by Laboratory of the Tianjin Chest Hospital. We used Gensini score to assess the severity of the coronary artery lesions. So we can analyze the relationship between plasma homocysteine and conventional risk factors of coronary heart disease, the distribution of MTHFR gene C677 T and A1298 C polymorphism on plasma homocysteine and the influence of gene polymorphism on the severity of the coronary artery lesions.Results:Expect for gender and age, hypertension, smoking, cholesterol had no significant influence on the plasma HCY levels. There was statistically significant differences in the average levels of plasma homocysteine of the coronary heart disease group( 15.39 + 8.59 mol / L) and the normal control group(12.89 + 5.38 mol / L, P < 0.001), HCY is a coronary heart disease independent risk factor(OR=1.035, P = 0.012).The severity of coronary artery disease of the HHCY group(mean rank =357.77) significantly higher than that of normal control group(mean rank =313.36). The percentage of MTHFR gene C677 T genotypes: TT genotype was 28.1%, CT genotype was 44.0%, CC genotype was 27.9%; percentage of MTHFR A1298 C genotypes: CC genotype was 1.9%, genotype AC 24.0%, AA genotype was 74.1%. MTHFR gene C677 T, plasma HCY level in the CC genotype was 11.91 + 7.91 mol/L, the level of plasma in CT genotype was 13.80 + 6.58 mol/L,the level of plasma in TT genotype was 18.84 + 9.72 mol/L, the difference between the each two groups were statistically significant(p<0.05); MTHFR gene in A1298 C, the plasma level of HCY in AA genotype group was 11.91 + 7.91 mol/L,the level of plasma HCY in AC genotype was 13.80 + 6.58 mol/L, the level of plasma HCY in CC genotype was 18.84 + 9.72 mol/L, there was no significant difference between the level of plasma HCY in AA genotype group and AC genotype group(p=0.256), but there were differences in the level of plasma HCY in CC genotype group and AA genotype, AC genotype(p=0.017, p=0.007). The level of plasma homocysteine in the MTHFR gene C677 T A1298C gene TTCC gene type was the highest(2.718 + 19.70 mol / L), the level of plasma HCY CCAA gene type was lowest(11.62 + 3.30 mol / L), The MTHFR gene C677 T TT genotype and MTHFR A1298 C CC genotype played jointly promote the role on the level of plasma HCY but the rest of the mutation genotype has not a significant role in promoting with other. The ratio of MTHFR gene C677 T genotype CC genotype in the coronary heart disease group was 19.2%, significantly lower than the normal control group, TT genotype in patients with coronary artery disease proportion was 30.4%, much higher than the normal control group the proportion(30.4%). There was no significant difference of the proportion of MTHFR gene C677 T group CT genotype and MTHFR A1298 C genotype AA, AC and CC genotype in the normal control group and the CHD group. The severity of coronary artery lesion in MTHFR C677 T genotype TT genotype group is the highest and the Gensini score in MTHFR A1298 C gene type in CC genotype was the highest.Conclusion: Expect for cholesterol and diabetes mellitus,gender age hypertension and smoking have effection on the level of plasma HCY;the mean plasma HCY levels in the CHD group is higher than normal control group, HCY is a independent risk factor for coronary heart disease.the HHCY group was significantly higher than the normal control group in the severity of the coronary artery lesions, MTHFR C677 T TT genotype and MTHFR gene A1298 C CC genotype have jointly promoted the level of plasma HCY; Among the rest,there was no genotype promote meaningful. MTHFR gene C677 T, A1298 C mutation is closely related to coronary heart disease. The susceptibility genotypes of coronary heart disease are MTHFR C677 T genotype TT genotype, CT genotype, MTHFR gene A1298 C each genotype CC genotype, AC type.