Whole Exome Capture Sequencing Identifies Candidate Genes Related To Colon Liver Metastasis

Objective:To study the key genes and the key pathways in the progress of colon cancer liver metastasis, have a more comprehensive understanding of the tumorigenesis and development of colon cancer liver metastasis and make a significant contribution to the early diagnosis and prognosis, we screened the mutant genes by the Whole Exon Sequencing technology and analyzed the mutant genes by the analysis tools in the public database. At the same time, we also wanted to explore a set of relatively efficient and simple method for screen the tumor associated genes, which can provide a methodological support for the discovery of animal tumor disease markers.Methods:1. We extracted the genomic DNA from the colon carcinoma tissues, paired metastatic liver focus tissues and paired normal colon tissues by using DNA purification kit. 2.we used the Whole Exon Sequencing technology to sequence the captured library independently. 3.We analyzed the mutant genes further by the methods of GO term analysis, KEGG Pathway analysis and protein interaction analysis.Results:1.27388 mutant genes were found in colon carcinoma tissues,27955 mutant genes were found in paired metastatic liver focus tissues,29126 mutant genes were found in paired normal colon tissues. Finally,372 mutant genes were found after removing the carcinoma tissues and normal tissues common mutant genes.2. The mutant genes concentrated in cell motion?cell adhesion?blood vessel development extracellular matrix component dynein complex?cytoskeletal part?basement membrane?notch signaling pathway?basal cell carcinoma?hedgehog signaling pathway? axon guidance.Conclusion:Notch signaling pathway?basal cell carcinoma?hedgehog signaling pathway and axon guidance regulated the colon cancer liver metastasis interlacedly rather than independently. And comprehensively thinking the GO?Pathway?protein interaction analysis results, we got 116 mutant genes associated with colon cancer liver metastases finally. We also builded a set of relatively efficient and simple method for screen the tumor associated genes, which can provide a methodological support for the discovery of animal tumor disease markers.