The Effects Of Wogonin On RET、pRET、GDNFR-α1 And CX3CR1 Expression Associated With PNI In Human Cervical Cancer Cells

Objective:The objective of this study is to investigate the effects of wogonin on PNI in human cervical cancer cells(Hela,CaSki and C33A) by studying the influence of the human cervical cells’migration ability and the expression of proteins(RET,pRET,GDNFR-al and CX3CR1) associated with PNI.Methods:1.The inhibitory effect of wogonin on the growth of human cervical cancer Hela,CaSki and C33A cells and the screening to concentration range of wogonin were investigated by using crystal violet assay.2.The migration of human cervical cancer Hela,CaSki and C33A cells after 12,24,48 hours of wogonin intervention was studied by using woud-healing assay.3.Western blot assay was used to detect the expression of RET,pRET,GDNFR-al and CX3CR1 in human cervical cancer Hela,CaSki and C33A cells induced by wogonin for 6 and 12 hours.Results:1. Wogonin could restrain the growth of human cervical cancer Hela,CaSki and C33A cells in dose-dependent manner and the 50% inhibitory concentration of these cells were (85.65±0.48505) μmol/L, (91.32±0.26963) μmol/L and (96.30±0.37242) μmol/L, respectively.The data showed the inhibitory effect of woginin on Hela cells is the most obvious,while the least on C33A cells (P<0.05).2.The migration of human cervical cancer Hela,CaSki and C33A cells were significantly inhibited by wogonin in vitro(P<0.05). Compared with the control group, the migration distances of Hela and CaSki cells after 12,24,48 hours of wogonin intervention were significantly reduced(P<0.05).No difference between the control group and C33A cells after 12 hours of wogonin intervention was observed(P>0.05).In C33A cells after 24 and 48 hours of wogonin intervention,the migration was obviously inhibited(P<0.05).3.Wogonin could down-regulate the expression of RET, pRET, GDNFR-al and CX3CR1 in human cervical cancer Hela, CaSki and C33A cells(P<0.05).The expression of GDNFR-al in Hela cells was down-regulated by wogonin in dose-dependent manner, and it was in in time-dependent manner in C33A cells (P<0.05). Wogonin inhibited the expression of pRET in C33A cells in both time and dose-dependent manner(P<0.05).Conclusion:1. The growth of human cervical cancer Hela,CaSki and C33A cells was inhibited by wogonin in vitro.2. Wogonin could inhibit the migration of human cervical cancer Hela,CaSki and C33A cells.3. Wogonin could inhibit the expression of RET,pRET,GDNFR-al and CX3CR1 in human cervical cancer Hela,CaSki and C33A cells.4. Wogonin might inhibit PNI by blocking GDNF-RET signal pathway and the expression of CX3CR1 in human cervical cancer cells.