Study On The Structure And Hemostatic Properties Of Silk Fibroin Peptide Prepared By Enzymatic Method/Ag-M58S

On the battlefield and everyday accidents,non-controlled bleeding is the leading cause of death.Therefore,it has been a hot topic to study new hemostatic materials and how to stop bleeding quickly and effectively.Silk fibroin is a kind of natural fiber material,which has the essential amino acids in human body and It is non-toxic,non-irritating and biodegradable.In addition,silk fibroin can also be processed into various kinds of materials according to the requirements,and can be modified by different chemical reagents.It is widely used in the field of textile industry and biomedicine.In order to further broaden the application of silk protein in the field of biomedical materials,this paper mainly studied the structure and hemostatic properties of silk fibroin peptide prepared by enzymatic method.Firstly,silk fibroin was hydrolyzed by Alcalase,Flavourzyme,Protamex and Pepsin.On this basis,silk fibroin was hydrolyzed by Alcalase and Flavourzyme,Protamex and Flavourzyme,and 6 groups of silk fibroin peptides were prepared.The morphology,structure and properties of 6 kinds of silk fibroin peptides were tested and characterized by FESEM,FTIR,XRD and Zeta potential tester.The hemostatic properties of silk peptide were evaluated by the blood viscosity test,platelet adhesion test,coagulation time in vitro.The hemostatic effect was confirmed by the model of rat liver hemostasis.Finally,in order to further improve the hemostatic properties of silk fibroin peptide hemostatic material.The silk fibroin peptide prepared by Alcalase was mixed with silver containing mesoporous Bioglass material by 5%,10%,15%,and 20% to prepare composite hemostatic material and studied on its structure and antibacterial property.Main research results show that:1.The results of in vitro blood tests and the model of rat liver hemostasis indicated that the 4 groups of silk fibroin peptides had certain hemostatic effect,and the hemostatic effect was better than that of gelatin sponge.Among them,the Alcalase group silk fibroin peptide had the best hemostatic effect,and the hemostatic effect of the Flavourzyme group silk fibroin peptide was similar to the Protamex group silk fibroin peptide,and the hemostatic effect of the Pepsin group silk fibroin peptide was poor.Since the 4 groups of silk fibroin peptides had the pore morphology,they contained random coil structure.The 4 groups of silk fibroin peptide had good water absorption and accelerated blood coagulation.At the same time,4 groups of silk fibroin peptide with negative potential,could quickly activate thrombin,shorten the clotting time,promote blood coagulation.2.The hemostatic effect of the silk fibroin peptide preparated by double enzyme was better than the silk fibroin preparated by single Flavourzyme.Because there were a lot of homogeneous and small pores on thesurface of silk fibroin peptide in AF group,the surface of silk fibroin peptide in PF group is larger pores.The two groups of silk fibroin peptide were mainly in the form of beta fold,including random coil structure.The preparation of silk fibroin peptide had good water absorption performance and with a more negative potential.This structure and properties improved blood viscosity,precipitated platelet aggregation on the surface of silk peptide,conducived to blood coagulation.3.Hemostatic and antibacterial properties of the composite hemostatic material were better than the silk fibroin peptide preparated by single Alcalase.After the addition of silver containing mesoporous Bioglass,the surface of the composite hemostatic material gradually became rough,the pores increased,the negative potential increased,and the coagulation and hemostasis effects were improved.With the increase of the content of Ag containing mesoporous Bioglass,the hemostatic antibacterial properties of the composites were improved.