Synthesis And Application Of PAMAM Based Nanomicelles And Cyanide Modified Carbon Nitride Nanomaterials

Gene therapy has gained significant attention over the past two decades as a potential method for treating genetic disorders such as severe combined immunodeficiency,cardiovascular diseases,and Parkinson's disease,as well as an alternative method to traditional chemotherapy used in treating cancer.As well,photodynamic therapy(PDT)is proven to be effective in superficial bladder cancer,early and obstructive lung cancer,Barrett ' s esophagus,head and neck cancers,and skin cancer.It is also been used as an adjunctive therapy following surgical resection of tumor,to reduce residual tumor burden.In the first part,we designed a novel amphiphilic dendrimer by conjugating reactive oxygen species(ROS)-responsive poly(propylenesulfide)(PPS)to low generation PAMAM dendrimer(G=2,Mw=3500 Da)via disulfide bond.This amphiphilic conjugate can self-assemble into stable nanomicelles.The results showed that the obtained nanomicelles have high zeta potential,which can condense DNA tightly.Moreover,the DNA/nanomicelle polyplexes can fully dissociate by intracellular ROS and easily release the entrapped DNA.As gene vector,the nanomicelles have realized high transfection efficacy and fairly low cytotoxicity in vitro.In the second part,we presented herein a simple and inexpensive system that,under solar irradiation,formed highly reductive radicals in the presence of an electron donor,with lifetimes exceeding the diurnal cycle.This radical species formed within a cyanamide-functionalized polymeric network of heptazine units and can give off its trapped electrons in the dark to generate ROS.KSCN-melon can be used as a photosensitizer to kill the cancer cells.