The Cultural Connotation Of Tibetan Medicine Bath And The Inflammatory Signaling Pathway Regulation Mechanism Of Wuwei Ganlu Yaoyu Granules On AA Rats Synovial Cells

Rheumatoid arthritis (RA), belonging to the Tibetan "zhen bu",is a common incurable disease in Tibetan areas.It has high incidence rate, high disability rate, and may involve multiple organs and systems disease.Tibetan medicine bath therapy is a common means of RA exact clinical effect, and "Wuwei Ganlu" prescription preparations are mainly used on the treatment of RA Tibetan medicine clinic. Tibetan medicine bath has become a national intangible cultural heritage protection project, and the State Administration of Traditional Tibetan medicine key specialty.At present, the research on Tibetan medicine bath "Wuwei Ganlu" mainly concentrated in the nursing in the clinical curative effect and the bath, medicine bath to adjust the immune system effective and high cure rate in the study is more extensive. But from the cell layer in the face of Tibetan medicine Wuwei Ganlu Yaoyu Granules to the lack of scientific and systematic research on the mechanism of the body, of the RA resistant molecular mechanism research is not in-depth.Purpose:From the theory of Tibetan medicine and the cultural connotation of the Tibetan medicine bath, Tibetan medicine bath in clinical experience as a guide, research on effect and Mechanism of Wuwei Ganlu Yaoyu Granules.From the whole animal model level,investigated the Pharmacodynamic action of Wuwei Ganlu Yaoyu Granules on the joint inflammation of RA rats; From the cellular level, analyzed the effects of cytokines on the expression of RA in the synovial tissue of rats;From the analysis of a biological level, screened related molecular information of RA, discussed regulation mechanism of Wuwei Ganlu Yaoyu Granules in RA synovial cell JAK-STAT inflammatory signaling pathway.Method:1.Cultural studies:From the perspective of medical anthropology, combined with the specific history of Tibetan medicine and Tibetan bathing culture background, integration of literature study, field survey and research methods to explain the cultural connotation of the interpretation of dipping.2.Experimental study:Established RA rat model and medicated bath treatment: ?The pro-inflammatory foot volume measurement of immune organ, the organ coefficient and ankle pathology morphology comparison,evaluated the rapeutic effect of Wuwei Ganlu Yaoyu Granules on AA rats;?Using enzyme-linked reaction, immunohistochemistry method to detect and analyze Wuwei Ganlu Yaoyu Granules intervention effect on the cytokines and the expression protein of Synovial tissue; ?Using gene chip technology, molecules expression was detected in synovial cells of JAK-STAT signal pathway related signaling, and by RT-PCR verification, discuss Wuwei Ganlu Yaoyu Granules in RA synovial cells of JAK-STAT signal pathway regulation mechanism.Result:1.Have more than a thousand years of history of Tibetan medicine bath contains rich culture of Tibetan bath, and in Tibetan medicine theory as a guide in clinical widely carried out. It set medical, health, health care and other functions in one, is the Tibetan external treatment with historical, cultural, economic and other multiple value.2.Wuwei Ganlu Yaoyu Granules in high dose group(11.80 g · L-1), middle dose group(5.90 g · L-1), low dose group(2.95 g · L-1)of AA rats primary foot swelling were relieved, having of varying degrees of improvement synovial cell hyperplasia, inflammatory cell infiltration, articular cartilage damage. Interleukin prime-1 beta (IL-1?), tumor necrosis factor alpha (TNF-a),growth factor (EGF,bFGF,PDGF-BB), prostaglandin E2 (PGE2),matrix metalloproteinases (MMP-3, TIMP-1)the content of in Serum varying degrees of decline.The drug can inhibit the activation of JAK-STAT signaling pathway in the synovial cells, so that the expression of P-STAT1, P-STAT3, IL-6 decreased,and the expression of 1L-10 increased;In the JAK-STAT signal pathway on I12rb, Mpl, Stam and other related signaling molecules up regulation, PIAS1,PIAS3,SOCS4,EPO,IFNGR2 and other related signaling molecules down.Conclusion:1.Tibetan medicine bath is the biological significance of science and anthropological sense of culture,with characteristics of both natural science and humanities.To fully understand the cultural connotation of Tibetan medicine,the humanistic culture and scientific culture in harmony.from the western science and technology means to carry out the modern study of Tibetan medicine bath.2.Tibetan medicine bath(Wuwei Ganlu Yaoyu Granules) in the process of rheumatoid arthritis synovial pathological changes, through down-regulated inflammatory cytokines (TNF-?, IL-1,6, etc.) and up-regulated anti-inflammatory cytokines (IL-10) to activate the body's immune system, and inhibit fibroblast-like synoviocytes to secrete of various cytokines to stimulation synovial, to control the synovial inflammation. The drug has a positive regulatory role in related signaling molecules expression in synovial cell JAK-STAT inflammatory signaling pathway, and then Further regulation of gene transcription in the nucleus, and finally achieve inhibition of RA synovial cell proliferation, promote apoptosis, anti-inflammation and detumescence, protect articular cartilage and bone.