| Photodynamic therapy(PDT)is an outstanding method to cancer therapy in recent years.Compared to surgery,chemotherapy and radiotherapy,the PDT has good selectivity,excellent tumor microvascular injury,less side effects,small trauma,low toxicity and repeatable treatment etc.However,the photosensitizer has poor water solubility,rapid metabolism by the human body and poor targeting et al.It is desired to develop a targeted anticancer drug nanocarriers with good biocompatibility and high loading efficiency for photosensitizer to overcome the above issue.Herein,calcium carbonate nanoparticles was selected as excellent drug carriers due to its good,biocompatibility,biodegradability and high loading efficiency for photosensitizer.Firstly,calcium carbonate particles with uniform nano size and porous structure were fabricated in gelatin-water/ethylene glycol mixture solution,which benefit the accumulation nanoparticles in tumor due to the enhanced permeability and retention(EPR)effect.Meanwhile the calcium carbonate nanoparticles can be dissolved in acidic solution,which avoid the potential risk of nanoparticles accumulation in human body in long term.Secondly,the surface of calcium carbonate nanoparticles was modified by hyaluronic acid to improve the thermodynamic stability in aqueous solution.Additionally,the surface modification by hyaluronic acid endows the nanoparticles with targeting property which selectively combines with the CD44 receptor overexpressed by cancer cells.To modify the surface of calcium carbonate nanoparticles,the hyaluronic acid was conjugated with cystamine through the method of EDC/NHS.After the reduction by DTT and cross-linked by PEGDA,astable hyaluronic acid layer was formed on the surface of calcium carbonate nanoparticles.The results showed that calcium carbonate nanoparticles could be targeted to the breast cancer cell MCF-7 with cell internalization efficiency about 85% after surface modification.In conclusion,the designed nanoparticles can be applied as an ideal drug carrier for photodynamic therapy. |
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