| Noise pollution has a centain impact on human and animals.Current research at home and abroad focuses on the impact of noise pollution on the hearing system,or comfined to one system,and the mechanism of noise pollution is lack of systematic research.Therefore,wistar rats were used as experimental subjects.Forty rats of male Wistar rats were randomly divided into 4 groups(n = 10).According to the noise of different decibels,the rats were divided into 38 dB group,55 dB group,70 dB group and control group,the control group was kept in normal environment.The experimental groups were given a corresponding noise stimulation at a fixed time each day for 30 minutes and continuously stimulated for 21 days.Rats were anesthetized with ether after the last stimulation,the rats were painlessly operated to open the chest,blood is collected from the left ventricle.The changes of blood component in rats were detected by automatic biochemical analyzer,the changes of cyclic guanosine monophosphate,cyclic adenosine monophosphate,heat shock protein-70,testosterone,dopamine and norepinephrine were measured by enzyme-linked immunosorbent assay(ELISA).The main results of this paper were as follows.Wistar rats were given 38 dB,55dB,70 dB stimulation for 21 days,the blood biochemical test results showed that,the levels of globulin,blood sugar,triglycerides and amylase in the blood of rats increased with the increase of noise decibels.The levels of globulin were increased by 6.07%,18.21% and 89.13% respectively compared with the control group.Blood glucose levels were increased by 502.67%,647.20% and 874.93%,respectively,compared with the control group.Triglyceride levels were increased by 5.54%,148.19% and 347.95%,respectively,compared with the control group.Amylase levels were increased by 18.59%,27.20% and 87.69%,respectively,compared with the control group.The contents of albumin,total bilirubin,creatine kinase,creatinine and urea in the blood of rats decreased with the increase of noise decibels.The levels of albumin were decreased by 6.47%,9.07% and 13.68%,respectively,compared with the control group.The total bilirubin level were 28.89%,44.63% and 71.85% lower than that of the control group,respectively.Creatine kinase levels were decreased by 3.48%,20.37% and 31.86%,respectively,compared with the control group.Creatinine levels were decreased by 2.75%,16.10% and 31.55%,respectively,compared with the control group.Urea levels were decreased by 17.51%,28.32% and 45.83%,respectively,compared with the control group.The results of the enzyme-linked immunosorbent assay showed that,The level of heat shock protein-70(HSP-70)in the blood of the experimental group was increased by 39.34%,195.09% and 285.25% respectively compared with the control group.The level of guanosine monophosphate(cGMP)was increased by 99.45%,115.71% and 171.40%,respectively,compared with the control group.The level of adenosine monophosphate(cAMP)was increased by 27.64%,132.49% and 270.79%,respectively,compared with the control group.Testosterone(T)levels were increased by 8.90%,22.87% and 35.80%,respectively,compared with the control group.The level of adrenaline(NA)increased by 34.12%,172.96% and 198.49%,respectively,compared with the control group.The levels of dopamine(DA)were increased by 11.33%,24.00% and 39.33%,respectively,compared with the control group.The above experimental results showed that Noise pollution can significantly affect blood component levels.The levels of amylase,blood glucose,triglyceride,cyclic adenosine monophosphate,cyclic guanosine monophosphate,heat shock protein-70,testosterone,dopamine and norepinephrine were increased with the increase of noise stimulation.The levels of creatine kinase,urea and creatinine in the blood were decreased with the increase of noise stimulation. |
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