| The liver regeneration has been studied for a long time,for it can provide theoretical basis for clinical treatment of liver diseases.The commonly used model of liver regeneration is 2/3 partial hepatectomy model.14-3-3 is a highly conserved protein with multiple functions.However,the study of these subtypes in rat liver regeneration has not been reported.During the liver regeneration,the changes of glucose metabolism is an important part in the study of liver regeneration,which can be reflected by the changes of enzymes in some key steps.As for cells in proliferation,14-3-3s are likely to affect the activities of enzymes in glucose metabolism by regulating some signaling molecules,and affect the glucose metabolism process as a result in rat liver regeneration.The purpose of the study was to provide theoretical basis for further study on the mechanism of liver regeneration by studying the effects of 14-3-3 and glucose metabolism related enzymes in rat the liver regeneration.Developed the experimental method for the above purposes.(1)Raised according to the Experimental Rat Raise Standard Operating Procedure,the SD-rats undergone partial hepatectomy operation;The SD-rat-livers were taken out at 0h,2h,6h,12 h,24h,36 h,72h,20 h,168h after the partial hepatectomy operation in order to prepare the samples yet to be detected,analysed by the radio of Cy3/Cy5.(2)In order to detect the expression of 7 kinds of 14-3-3 proteins,14-3-3?/?,14-3-3?,14-3-3?,14-3-3?,14-3-3?,14-3-3?/?,14-3-3?/?,and the carbohydrate-associated enzyme genes in rat liver regeneration.,the Rat Genome 230 2.0 Array and Western Blot were used.(3)The expression of the 6 kinds of carbohydrate-associated enzyme genes were detected by RT-PCR,analysed by 2-??ct and T-Test.Finally,the experimental results were obtained.(1)In the 168 h after partial hepatectomy in rats,there were 4 types,14-3-3?,14-3-3?,14-3-3?,14-3-3?/?,expressed in the 7 kinds of 14-3-3 proteins.Compared with the expression at 0h,the expression of 14-3-3? at 2h was down-regulated(t<t0.05(4)=2.776,p>0.05),and the expressions at 24 h,30h,72 h,120h and 168 h were significantly up-regulated(t=3.871,2.970,2.895,2.970,3.899>t0.05(4)=2.776,p<0.05);the expression of 14-3-3? at 30 h was up-regulate-d(t=5.750>t0.01(4)=4.601,p<0.01);the expressions of 14-3-3?/? at 2h,24 h,30h and 36 h were up-regulat ed(t=3.948,3.660,3.952,2.860>t0.05(4)=2.776,p<0.05).Compared with the expression at 0h,the expression s of 14-3-3 proteins showed no significant differences at the other time points(t<t0.05(4)=2.776,p>0.05).(2)Compared with the expression at 0h,the expressions of Pyruvate Kinase LR at 2h was signif ic-antly up-regulated(P<0.01),and that at 12 h,24h,30 h,36h and 120 h were significantly down-regulate d(P<0.01),and at 6h,72 h,168h were significantly down-regulated(P<0.05);the expressions of Pyruvat e Dehydrogenase HA at 6h was significantly down-regulated(P<0.05),and that at 12 h,30h,36 h,72h,120 h and 168 h were significantly down-regulated(P<0.01);the expressions of Citrate Synthase at 6h,12 h,30h,36 h,72h and 168 h were significantly down-regulated(P<0.01);the expressions of Lactate Dehyd r-ogenase A at 2h,6h,12 h,24h,36 h,72h and 120 h were significantly down-regulated(P<0.01);the expre s-sions of Lactate Dehydrogenase B at 2h was significantly up-regulated(P<0.01);the expressions of Glucose-6-Phosphate Dehydrogenase at 2h was significantly down-regulated(P<0.05),and that at 6h,12 h,24h,30 h,36h,72 h and 168 h were significantly down-regulated(P<0.01).Compared with the express-ion at 0h,the expressions at the other time points showed no significant differences(P>0.05).Experimental conclusion:(1)During the rat liver regeneration,14-3-3? might be involved in the G1/M phase while 14-3-3?,14-3-3? and 14-3-3? might be involved in the G2/S phase and 14-3-3? might participate in the regulation of cell cycle termination,whereas there were only 4 subtypes,14-3-3?,14-3-3?,14-3-3?,14-3-3?,in translation.(2)Carbohydrate metabolism was closely related to the ratliver regeneration.(3)14-3-3?,14-3-3?,14-3-3? and 14-3-3?/? might affect carbohydrate metabolism during rat liver regeneration. |
PDF Full Text Request